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Titolo:
BIOSYNTHESIS OF GROWTH HORMONE-RELEASING FACTOR BY FETAL-RAT CEREBROCORTICAL AND HYPOTHALAMIC CELLS
Autore:
VAZQUEZ GF; CACICEDO L; LORENZO MJ; DELOSFRAILES MT; LARA JI; FRANCO FS;
Indirizzi:
INST SALUD CARLOS 3,CTR NACL INVEST CLIN & MED PREVENT,SERV ENDOCRINOL,SINESIO DELGADO 10 E-28029 MADRID SPAIN INST SALUD CARLOS 3,CTR NACL INVEST CLIN & MED PREVENT,SERV ENDOCRINOL E-28029 MADRID SPAIN HOSP RAMON Y CAJAL,SERV ENDOCRINOL MADRID SPAIN FDN JIMENEZ DIAZ,SERV ENDOCRINOL MADRID SPAIN
Titolo Testata:
Peptides
fascicolo: 5, volume: 15, anno: 1994,
pagine: 825 - 828
SICI:
0196-9781(1994)15:5<825:BOGHFB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-LIKE IMMUNOREACTIVITY; TUMOR;
Keywords:
GRF BIOSYNTHESIS; CEREBROCORTICAL AND HYPOTHALAMIC CELLS; PRIMARY CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
G.F. Vazquez et al., "BIOSYNTHESIS OF GROWTH HORMONE-RELEASING FACTOR BY FETAL-RAT CEREBROCORTICAL AND HYPOTHALAMIC CELLS", Peptides, 15(5), 1994, pp. 825-828

Abstract

The biosynthesis of growth hormone-releasing factor (GRF) by cerebrocortical tissue is controversial. Although several reports have indicated its presence in certain rat cortical areas and in cultured rat hypothalamic cells, no data exist demonstrating its biosynthesis in these areas. In this study, we have investigated the capacity of fetal rat cerebrocortical and hypothalamic cells in culture for synthesizing GRF. Fetal cerebrocortical and hypothalamic cells were exposed to [H-3]Argfor 48 h. Medium and cell extracts were processed and [H-3]Arg-IR-rGRF was isolated by affinity chromatography and characterized by HPLC. Intracellular [H-3]Arg-IR-rGRF from both hypothalamic and cerebrocortical cells exhibited four major peaks, one of them coeluting with synthetic rGRF. In cerebrocortical cultures, newly synthesized and released [H-3]Arg-IR-rGRF showed a similar pattern to the cell content. However, in media from hypothalamic cells, higher hydrophobicity molecular forms were absent. The data demonstrated that fetal cerebrocortical and hypothalamic cells in primary culture synthesize GRF with similar posttranslational processing, but with different molecular patterns of secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:09:25