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Titolo:
DUAL-CHANNEL CONFOCAL LASER-SCANNING MICROSCOPY OF LUCIFER YELLOW-MICROINJECTED HUMAN BRAIN-CELLS COMBINED WITH TEXAS RED IMMUNOFLUORESCENCE
Autore:
BELICHENKO PV; DAHLSTROM A;
Indirizzi:
GOTHENBURG UNIV,INST NEUROBIOL NRCG,DEPT ANAT & CELL BIOL,MEDICINAREGATAN 5 S-41390 GOTHENBURG SWEDEN GOTHENBURG UNIV,INST NEUROBIOL NRCG,DEPT ANAT & CELL BIOL S-41390 GOTHENBURG SWEDEN RUSSIAN ACAD MED SCI,BRAIN RES INST MOSCOW RUSSIA
Titolo Testata:
Journal of neuroscience methods
fascicolo: 2, volume: 52, anno: 1994,
pagine: 111 - 118
SICI:
0165-0270(1994)52:2<111:DCLMOL>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOAMINE OXIDASE-A; NEURONS; IDENTIFICATION; INJECTION; ANTIBODIES; RECEPTOR; CLONING; CDNA;
Keywords:
LUCIFER YELLOW; INTRACELLULAR STAINING; IMMUNOFLUORESCENCE; DOUBLE LABELING; CONFOCAL LASER SCANNING MICROSCOPY; HUMAN BRAIN MAPPING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
P.V. Belichenko e A. Dahlstrom, "DUAL-CHANNEL CONFOCAL LASER-SCANNING MICROSCOPY OF LUCIFER YELLOW-MICROINJECTED HUMAN BRAIN-CELLS COMBINED WITH TEXAS RED IMMUNOFLUORESCENCE", Journal of neuroscience methods, 52(2), 1994, pp. 111-118

Abstract

A method for visualization of individual human brain cells and their dendritic extensions in combination with immunofluorescence is described. Microinjection of Lucifer Yellow was used to reveal the dendritic morphology of cortical brain cells. Indirect immunofluorescence with Texas Red as label was used to investigate the distribution of 3 different groups of immunogens: enzymes (monoamine oxidase A and B), receptors (beta-adrenoceptor protein), and synaptic vesicle proteins (synapsin I and synaptophysin) in each cortical slice. A dual-channel confocallaser scanning microscope with an argon/krypton laser was used for imaging these double-stained fluorescent specimens. Lucifer Yellow and Texas Red were recorded simultaneously or separately, taking advantage of the different activating lines (488 lambda and 568 lambda) of the laser and using the two filter blocks (K1 and K2) supplied with the instrument (BioRad MRC-600) for recording the emission of either fluorophore. Using this technique we have demonstrated the localization of immunoreactive material in relatation to the dendritic morphology of cortical cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:55:28