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Titolo:
COMPARISON OF ENHANCED CHEMILUMINESCENCE AND COLORIMETRIC TECHNIQUES FOR THE IMMUNO-DETECTION OF ALPHA(1)-ANTITRYPSIN
Autore:
VENEMBRE P; SETA N; BOUTTEN A; DEHOUX M; AUBIER M; DURAND G;
Indirizzi:
HOP BICHAT,BIOCHIM LAB A,46 RUE H HUCHARD F-75877 PARIS 18 FRANCE HOP BICHAT,INSERM 408,SERV PNEUMOL F-75877 PARIS 18 FRANCE
Titolo Testata:
Clinica chimica acta
fascicolo: 1-2, volume: 227, anno: 1994,
pagine: 175 - 184
SICI:
0009-8981(1994)227:1-2<175:COECAC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MONOCYTES; DEFICIENCY; PROTEINS;
Keywords:
WESTERN-BLOT; DOT-BLOT; ECL; CELL CULTURE SUPERNATANT; BIOLOGICAL FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
P. Venembre et al., "COMPARISON OF ENHANCED CHEMILUMINESCENCE AND COLORIMETRIC TECHNIQUES FOR THE IMMUNO-DETECTION OF ALPHA(1)-ANTITRYPSIN", Clinica chimica acta, 227(1-2), 1994, pp. 175-184

Abstract

In order to detect, characterize and quantify blotted proteins, such as human alpha(1)-antitrypsin (AAT), there is a need for a specific, extremely sensitive, non-radioactive and uniform revelation system applicable to diluted biological fluids and to culture supernatants of cells isolated from such fluids. We compared two immunochemical revelation systems, enhanced chemiluminescence (ECL) and colorimetric procedures, applied to human AAT, after determining their optimal conditions ofperformance. ECL was the most sensitive method (down to 50 pg blottedAAT), but could not be used to quantify AAT. In contrast, the colorimetric method enables quantification of blotted AAT, either simply dotted or transferred after SDS-polyacrylamide gel electrophoresis, but isnot as sensitive as ECL. Using these two complementary procedures, wehave been able to detect AAT in the culture supernatant of a monocytic cell line (THP-1), to characterize the different forms of AAT present in the culture supernatant of blood monocytes and to quantify both.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:56:28