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Titolo:
INTERPRETING THE EFFECTS OF SITE-DIRECTED MUTAGENESIS ON ACTIVE-TRANSPORT SYSTEMS
Autore:
KRUPKA RM;
Indirizzi:
AGR CANADA,LONDON RES CTR,1391 SANDFORD ST LONDON N5V 2T3 ON CANADA
Titolo Testata:
Biochimica et biophysica acta. Biomembranes
fascicolo: 1, volume: 1193, anno: 1994,
pagine: 165 - 178
SICI:
0005-2736(1994)1193:1<165:ITEOSM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; COUPLING MECHANISMS; COA DECARBOXYLASE; SODIUM-TRANSPORT; CALCIUM-PUMP; TRANSLOCATION; PURIFICATION; EXCHANGE; BACTERIA; PROTEIN;
Keywords:
UNCOUPLED TRANSPORT; COTRANSPORT; MUTATION; LACTOSE PERMEASE; CALCIUM PUMP; TRANSPORT KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Krupka, "INTERPRETING THE EFFECTS OF SITE-DIRECTED MUTAGENESIS ON ACTIVE-TRANSPORT SYSTEMS", Biochimica et biophysica acta. Biomembranes, 1193(1), 1994, pp. 165-178

Abstract

Single amino acid substitutions in the lactose permease of Escherichia coli are known to elicit behaviour, such as the transformation of anactive into a passive system, not explained by current co-transport models. The behaviour, it is shown, can be explained by an expanded reaction scheme that takes account of the required alternation of the carrier, in the course of the coupled reaction, between mobile and immobile conformations or between conformations that bind either only one substrate or both substrates. The extended model links such behaviour toaltered conformational equilibria or binding regions. Thus, mutationsthat affect the equilibrium between a mobile one-site conformation ofthe free carrier and an immobile conformation having sites for both substrates allow passive transport of the second substrate in an ordered mechanism, and mutations in a secondary substrate binding region that affects this conformational change allow passive transport of the first substrate. Mutations in regions interacting with a substrate in the transition state in carrier movement, as well as in the initial binding sites, can also be distinguished. The analysis applies to both primary and secondary active transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:36:06