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Titolo:
IDENTIFICATION OF A HIGH-MOLECULAR-WEIGHT CELLULAR PROTEIN COMPLEX CONTAINING THE ADENOVIRUS DNA-BINDING PROTEIN
Autore:
RICIGLIANO JW; BROUGH DE; KLESSIG DF;
Indirizzi:
RUTGERS STATE UNIV,WAKSMAN INST MICROBIOL,POB 759 PISCATAWAY NJ 08855 RUTGERS STATE UNIV,WAKSMAN INST MICROBIOL PISCATAWAY NJ 08855
Titolo Testata:
Virology
fascicolo: 2, volume: 202, anno: 1994,
pagine: 715 - 723
SICI:
0042-6822(1994)202:2<715:IOAHCP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONKEY CELLS; MESSENGER-RNA; HELA-CELLS; LATE GENES; TRANSCRIPTION; REPLICATION; MUTATIONS; EXPRESS; NFI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.W. Ricigliano et al., "IDENTIFICATION OF A HIGH-MOLECULAR-WEIGHT CELLULAR PROTEIN COMPLEX CONTAINING THE ADENOVIRUS DNA-BINDING PROTEIN", Virology, 202(2), 1994, pp. 715-723

Abstract

The adenovirus DNA binding protein (DBP) functions in a number of diverse processes that include viral DNA replication and host range determination. A subpopulation of DBP has been found to exist in a high-molecular-weight (HMWDBP) complex of greater than 650 kDa. This complex was stable to high salt and low levels of detergent in the presence of DTT at 4 degrees. While the complex prepared in high salt was devoid of nucleic acid, it retained its ability to bind to ssDNA. Electron microscopic (EM) analysis in the presence of M13 ssDNA verified its interaction with ssDNA. The complex exists as particles approximately 25-30nm based on EM analysis of negatively stained samples in the absence of DNA. No viral replication components were detected in the HMWDBP complex, suggesting that it is not a viral replication complex. The HMWDBP complex prepared under a variety of infection conditions contained a consistent set of proteins. HMWDBP complex was also detected in the absence of viral infection in gmDBP6 cells (a HeLa cell derivative that expresses DBP from the dexamethasone-inducible MMTV promoter). Theseresults suggest that the components of the complex, other than DBP, are host encoded. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:50:53