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Titolo:
QUANTITATIVE MICRO P-30 AND REVERSE-TRANSCRIPTASE ASSAYS FOR MOLONEY MURINE LEUKEMIA-VIRUS
Autore:
WILSON CA; EIDEN MV; MARSH JW;
Indirizzi:
NIMH,MOLEC BIOL LAB,BLDG 36,ROOM 1D03 BETHESDA MD 20892 NIMH,MOLEC BIOL LAB BETHESDA MD 20892 NIMH,CELL BIOL LAB BETHESDA MD 20892
Titolo Testata:
Journal of virological methods
fascicolo: 1, volume: 48, anno: 1994,
pagine: 109 - 117
SICI:
0166-0934(1994)48:1<109:QMPARA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLAQUE ASSAY;
Keywords:
REVERSE TRANSCRIPTASE; RETROVIRUS; MOMLV; MOLONEY MURINE LEUKEMIA VIRUS; P-30;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
6
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Wilson et al., "QUANTITATIVE MICRO P-30 AND REVERSE-TRANSCRIPTASE ASSAYS FOR MOLONEY MURINE LEUKEMIA-VIRUS", Journal of virological methods, 48(1), 1994, pp. 109-117

Abstract

An anti-P30 immunohistochemical and a reverse transcriptase assay forMoloney murine leukemia virus (MoMLV) are adapted to 96-well plates. The assay results are shown to be directly proportional to the number of infectious particles, and can therefore be used to estimate the infective titers of a virus preparation. The micro P30 assay yields a direct estimate of infectious centers; and the reverse transcriptase assay quantitates progeny from a single cycle of replication. The semi-automated nature of these assays is well suited to the analysis of a large number of samples and therefore permits the examination of the efficiency of the process of retroviral/MoMLV infection under varied times or conditions.

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Documento generato il 30/05/20 alle ore 08:07:46