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Titolo:
CORTICAL STIMULATION INDUCES FOS EXPRESSION IN INTRASTRIATAL STRIATALGRAFTS
Autore:
LABANDEIRAGARCIA JL; GUERRA MJ;
Indirizzi:
UNIV SANTIAGO COMPOSTELA,FAC MED,DEPT MORPHOL SCI E-15705 SANTIAGO SPAIN
Titolo Testata:
Brain research
fascicolo: 1, volume: 652, anno: 1994,
pagine: 87 - 97
SICI:
0006-8993(1994)652:1<87:CSIFEI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-LESIONED STRIATUM; UNILATERAL NEOSTRIATAL LESIONS; HOST DOPAMINERGIC AFFERENTS; HUNTINGTONS-DISEASE; NERVOUS-SYSTEM; RAT STRIATUM; ADULT-RATS; NEURONS; ORGANIZATION; PRIMORDIA;
Keywords:
GRAFT; STRIATUM; CORTICOSTRIATAL AFFERENT; TRANSPLANTATION; POSTSYNAPTIC RESPONSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Labandeiragarcia e M.J. Guerra, "CORTICAL STIMULATION INDUCES FOS EXPRESSION IN INTRASTRIATAL STRIATALGRAFTS", Brain research, 652(1), 1994, pp. 87-97

Abstract

Innervation of intrastriatal grafts of fetal striatal tissue by host corticostriatal projections has been shown in a number of previous studies in rats. In the work reported here, induction of Fos protein in grafted striatal neurons by electrical stimulation of the host frontoparietal cortex has been used as cell-level marker of corticostriatal postsynaptic responses within the striatal grafts. Unilateral cortical stimulation 30 min before sacrifice led to bilateral widespread and intense Fos induction throughout the normal striatum, although the response was somewhat more intense ipsilaterally and in the dorsolateral rostral striatum. In adult rats whose striatum had been lesioned with ibotenic acid 10-12 days prior to implantation of fetal striatal tissue, 3- and 18-month-old striatal grafts showed Fos immunoreactivity in a considerable number of cells after either bilateral, or ipsilateral (similar to 30-40% of the density of Fos-immunoreactive cells in the normal striatum) or contralateral cortical stimulation. Double-Fos and -DARPP-32 immunohistochemistry revealed that the Fos-immunoreactive nuclei were concentrated in the DARPP-32-positive (i.e. striatum-like) patches, which contained similar to 60% of the density of Fos-positive nuclei in the normal striatum after either ipsilateral or bilateral stimulation. However, Fos-immunoreactive nuclei were unevenly distributed within the DARPP-32-positive compartment of the graft, with some clusters of Fos-immmunoreactive nuclei at 2-3 X the density observed in the normal striatum and other areas with Fos-immunoreactive nuclei presentat lower density or absent. Fos induction was also observed in 4-week-old grafts, indicating that functional corticostriatal synaptic contacts develop rapidly. Striatal grafts implanted either in non-lesioned host striatum or in long-term (18 months) lesioned striatum, similarlyshowed Fos-positive nuclei after cortical stimulation, indicating that host corticostriatal fibers are equally capable of establishing functional synaptic contacts under these conditions. These results indicate that host corticostriatal fibres not only form an axonal network within the graft but also induce postsynaptic responses which may contribute to the observed graft-induced amelioration of lesion-derived behavioural deficits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:26:45