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Titolo:
ELASTASE AND ALPHA-1-PROTEINASE INHIBITOR IN GINGIVAL CREVICULAR FLUID AND GINGIVAL TISSUE IN ADULT AND JUVENILE PERIODONTITIS
Autore:
INGMAN T; SORSA T; KANGASPUNTA P; KONTTINEN YT;
Indirizzi:
UNIV HELSINKI,DEPT ANAT,POB 9,SILTAVUORENPENGER 20A SF-00014 HELSINKIFINLAND UNIV HELSINKI,DEPT PERIODONTOL SF-00100 HELSINKI 10 FINLAND UNIV HELSINKI,DEPT MED CHEM SF-00100 HELSINKI 10 FINLAND
Titolo Testata:
Journal of periodontology
fascicolo: 7, volume: 65, anno: 1994,
pagine: 702 - 709
SICI:
0022-3492(1994)65:7<702:EAAIIG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN NEUTROPHIL ELASTASE; TETRACYCLINE INHIBITION; LEUKOCYTE ELASTASE; SURGICAL-TREATMENT; COLLAGENASE; DEGRADATION; PROTEINASES; DISEASES; GRANULES; ANTIBODY;
Keywords:
ELASTASE; GINGIVITIS IMMUNOLOGY; GINGIVAL CREVICULAR FLUID ANALYSIS; PERIODONTITIS, JUVENILE IMMUNOLOGY; PERIODONTITIS IMMUNOLOGY; PROTEINASE INHIBITORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
T. Ingman et al., "ELASTASE AND ALPHA-1-PROTEINASE INHIBITOR IN GINGIVAL CREVICULAR FLUID AND GINGIVAL TISSUE IN ADULT AND JUVENILE PERIODONTITIS", Journal of periodontology, 65(7), 1994, pp. 702-709

Abstract

THE PRESENCE AND LOCALIZATION of PMN/neutrophil elastase and its endogenous inhibitor alpha1-proteinase inhibitor (alpha1PI) were studied immunohistochemically in gingival tissue specimens collected from 9 adult periodontitis (AP) patients during flap surgery after the initial phase of periodontal therapy, and from 6 healthy controls with clinically-healthy periodontium upon surgical extraction of impacted third molars. In order to evaluate how periodontal tissue destructive events are reflected in gingival crevicular fluid (GCF), GCF samples were collected from the AP patients before any periodontal treatment and prior to flap surgery, from 5 localized juvenile periodontitis (LJP) patients, and from the controls. Elastase activity in the GCF was measured with the SAAVNA-assay and the molecular forms and amount of alpha1PI by Western- and dot-blotting. Immunohistochemical staining for PMN elastase was strongly positive in the connective tissue, but not in the epithelium, of the AP patients' gingival tissue specimens. In the healthy gingival tissue specimens only a few elastase-positive cells were present. Both in AP and in control gingival specimens, alpha1PI was detected in the connective tissue and in the keratinized layer of the epithelium, however, its amount was markedly lower in the control specimens. Elevated levels of alpha1PI and PMN elastase were detected in the GCF of all periodontitis patients when compared to controls. Based on the present findings we conclude that: 1) PMN elastase activity is associated with periodontal inflammation in situ, and increased activities are detected in AP GCF, but not in LJP GCF, and 2) alpha1PI is present both in healthy and in inflamed periodontal tissues, but during periodontal inflammation its amount is increased, probably due to a compensatory extracellular matrix (ECM) -protective mechanisms of the host's defense system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:02:21