Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIFFERENTIAL GROWTH-FACTOR INDUCED MODULATION OF PROTEOGLYCANS SYNTHESIZED BY NORMAL HUMAN RENAL VERSUS CYST-DERIVED CELLS
Autore:
KOVACS J; CARONE FA; LIU ZZ; NAKUMARA S; KUMAR A; KANWAR YS;
Indirizzi:
NORTHWESTERN UNIV,SCH MED,DEPT PATHOL,303 E CHICAGO AVE CHICAGO IL 60611 NORTHWESTERN UNIV,SCH MED,DEPT PATHOL,303 E CHICAGO AVE CHICAGO IL 60611
Titolo Testata:
Journal of the American Society of Nephrology
fascicolo: 1, volume: 5, anno: 1994,
pagine: 47 - 54
SICI:
1046-6673(1994)5:1<47:DGIMOP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYSTIC KIDNEY-DISEASE; DECREASED DENOVO SYNTHESIS; BASEMENT-MEMBRANE; CPK MOUSE; GLOMERULAR PROTEOGLYCANS; PROTOONCOGENE EXPRESSION; SULFATE PROTEOGLYCANS; MESSENGER-RNA; CULTURES; INVITRO;
Keywords:
GROWTH FACTORS; PROTEOGLYCANS; POLYCYSTIC KIDNEY DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
J. Kovacs et al., "DIFFERENTIAL GROWTH-FACTOR INDUCED MODULATION OF PROTEOGLYCANS SYNTHESIZED BY NORMAL HUMAN RENAL VERSUS CYST-DERIVED CELLS", Journal of the American Society of Nephrology, 5(1), 1994, pp. 47-54

Abstract

In polycystic kidney disease (PKD), there is an insiduous enlargementof the kidneys and dilation of the renal tubules associated with extracellular matrix (ECM) alterations. The latter include thickening of tubular basement membrane and decreased synthesis of sulfated proteoglycan (PG). Because PKD is believed to be a disorder of cell growth and deranged ECM metabolism, it is conceivable that the formation of cystic tubules may be modulated by certain growth factors (GF) that influence the synthesis of ECM glycoproteins. In this study, the effect of various GF, i.e., epidermal, hepatocyte (HGF) and transforming (TGF), and triiodothyronine on the PG synthesized by normal human kidney (NK) epithelial cells and cells derived from cysts of patients with autosomal dominant PKD (ADPKD) was assessed. (S-35) sulfate incorporation studies revealed that, among various GF, HGF and TGF-beta1 had the maximalstimulatory effect on the synthesis of PG extracted from ADPKD cells,A minimal increase in the PG synthesis was observed in NK cells; however, PG synthesized under the influence of HGF or TGF-beta1 were of relatively higher molecular weight, with a shift of K(av) from 0.28 to 0.12, as ascertained by Sepharose-6B chromatography. PG synthesized by ADPKD cells had a K(av) = 0.18, and it did not change with the GF treatment. The charge-density characteristics of PG of ADPKD cells were relatively lower than those of NK cells, and they were unaffected by HGFor TGF-beta1 treatment. Interestingly, both the HGF and TGF-beta1 significantly affected posttranslational modifications of PG. The HGF preferentially enhanced the synthesis of heparan sulfate-PG in both cell types, whereas TGF-beta1 disproportionately stimulated the synthesis of chondroitin sulfate-PG. These data indicate that HGF and TGF-beta1 selectively modulate the synthesis of PG derived from ADPKD cells, whereas the GF differentially influence the posttranslational modifications of PG in both the NK and ADPKD cells, henceforth, suggesting a potential role of GF in the pathogenesis of PKD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:30:24