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Titolo:
INTERACTIONS OF HERBICIDES 2,4-D AND DINOSEB WITH LIVER MITOCHONDRIALBIOENERGETICS
Autore:
PALMEIRA CM; MORENO AJ; MADEIRA VMC;
Indirizzi:
UNIV COIMBRA,DEPT ZOOL P-3049 COIMBRA PORTUGAL UNIV COIMBRA,DEPT BIOCHEM P-3049 COIMBRA PORTUGAL
Titolo Testata:
Toxicology and applied pharmacology
fascicolo: 1, volume: 127, anno: 1994,
pagine: 50 - 57
SICI:
0041-008X(1994)127:1<50:IOH2AD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE-PHOSPHORYLATION; RESPIRATION; RAT; MEMBRANE; INVITRO; INVIVO; CANCER; STATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Palmeira et al., "INTERACTIONS OF HERBICIDES 2,4-D AND DINOSEB WITH LIVER MITOCHONDRIALBIOENERGETICS", Toxicology and applied pharmacology, 127(1), 1994, pp. 50-57

Abstract

The herbicides 2,4-D (2,4-dichlorophenoxyacetic acid) and dinoseb (2-sec-butyl-4,6-dinitrophenol), were tested in mitochondria because theyare putative toxins to the organisms. To understand the toxic mechanisms involved, we have determined if mitochondrial bioenergetic functions are affected. Dinoseb partially inhibits uncoupled respiration, reflecting its limited interaction with the mitochondrial redox chain at the level of succinate dehydrogenase and cytochrome c reductase (complex III). Additionally, it increased the rate of state 4 oxygen consumption, stimulated ATPase activity, induced permeabilization of membranemitochondria to H+, and depressed Delta Psi. These data characterize dinoseb as a classical proton uncoupler. The herbicide 2,4-D decreasedDelta Psi as a function of concentration and the rate of repolarization was also progressively decreased. State 3 and uncoupled respirationwere depressed by approximately the same extent (60%), ruling out interactions on phosphorylation assembly independent of the redox chain. The herbicide strongly inhibited succinate dehydrogenase and cytochrome c reductase (complex III), whereas cytochrome c oxidase was not affected. Additionally, 2,4-D also uncoupled mitochondria at concentrations 1000-fold higher than those required for a similar dinoseb effect. This study therefore suggests that dinoseb- and 2,4-D-induced cellular damage, as we have reported before, is putatively preceded by injury upon bioenergetic functions of mitochondria. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:20:30