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Titolo:
CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE-V - TISSUE DISTRIBUTION AND IMMUNOHISTOCHEMICAL LOCALIZATION IN RAT-BRAIN/
Autore:
ITO T; MOCHIZUKI H; KATO M; NIMURA Y; HANAI T; USUDA N; HIDAKA H;
Indirizzi:
NAGOYA UNIV,SCH MED,DEPT PHARMACOL,SHOWA KU NAGOYA AICHI 466 JAPAN NAGOYA UNIV,SCH MED,DEPT PHARMACOL,SHOWA KU NAGOYA AICHI 466 JAPAN NAGOYA UNIV,SCH MED,DEPT SURG 1,SHOWA KU NAGOYA AICHI 466 JAPAN SHINSHU UNIV,SCH MED,DEPT ANAT MATSUMOTO NAGANO 390 JAPAN
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 1, volume: 312, anno: 1994,
pagine: 278 - 284
SICI:
0003-9861(1994)312:1<278:CCP-TD>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALMODULIN-DEPENDENT KINASE; MR 100,000 SUBSTRATE; PURIFICATION; IDENTIFICATION; CEREBELLUM; FOREBRAIN; ANTIBODY; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
T. Ito et al., "CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE-V - TISSUE DISTRIBUTION AND IMMUNOHISTOCHEMICAL LOCALIZATION IN RAT-BRAIN/", Archives of biochemistry and biophysics, 312(1), 1994, pp. 278-284

Abstract

Polyclonal antibody against Ca2+/calmodulin-dependent protein kinase V (CaM kinase V) was prepared from guinea pigs immunized with synthetic polypeptide, based on the partial amino acid sequence of the rat brain enzyme. Immunoblot analysis of purified CaM kinase V revealed two immunoreactive bands with a molecular mass of 41 kDa and minor 40 kDa, respectively. Tissue distribution of CaM kinase V and immunohistochemical localization in rat brain were also investigated. Immunoblotting revealed the presence of immunoreactive proteins with the same molecular mass of 40 and 41 kDa, in the cerebrum, cerebellum, brain stem, pituitary gland, lung, adrenal gland, spleen, liver, colon, heart, stomach, ovary, spinal cord, and thymus. Immunohistochemistry revealed strongstaining in the neuronal somata and weak staining in the nuclei. Densely stained regions included the cerebral cortex, the hippocampal formation, the caudatoputamen, the globus pallidus, the hypothalamus, the substantia nigra, the medial geniculate body, the olfactory bulb, the cerebellar cortex and the choroid plexus. CaM kinase V revealed different substrate specificity compared with CaM kinase II. These results lead to the notion that CaM kinase V may exist in 40- and 41-kDa isoforms, is widely distributed in various tissues, and may play an important role in the control of a wide variety of calcium regulated processesin the respective tissues. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:51:18