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Titolo:
IN-VITRO BIOCOMPATIBILITY OF ALTERNATIVE CAPD FLUIDS - COMPARISON OF BICARBONATE-BUFFERED AND GLUCOSE-POLYMER-BASED SOLUTIONS
Autore:
JORRES A; GAHL GM; TOPLEY N; NEUBAUER A; LUDAT K; MULLER C; PASSLICKDEETJEN J;
Indirizzi:
UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,STANDORT CHARLOTTENBURG,DEPT NEPHROL,SPANDAUER DAMM 130 D-14050 BERLIN GERMANY UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,DEPT CLIN BIOCHEM D-14050 BERLIN GERMANY UNIV WALES COLL CARDIFF,ROYAL INFIRM,COLL MED,INST NEPHROL CARDIFF CF1 3NS S GLAM WALES FRESENIUS AG OBERURSEL GERMANY UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,DEPT HEMATOL D-14050 BERLIN GERMANY
Titolo Testata:
Nephrology, dialysis, transplantation
fascicolo: 7, volume: 9, anno: 1994,
pagine: 785 - 790
SICI:
0931-0509(1994)9:7<785:IBOACF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; PERITONEAL-DIALYSIS FLUID; FACTOR TNF; INTERLEUKIN-6; CELLS; INHIBITION; MACROPHAGES; LEUKOCYTES; RELEASE; IL-6;
Keywords:
BIOCOMPATIBILITY; CAPD FLUIDS; CONTINUOUS AMBULATORY PERITONEAL DIALYSIS; CYTOKINES; IL-6; POLYMERASE CHAIN REACTION; TNF(ALPHA);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Jorres et al., "IN-VITRO BIOCOMPATIBILITY OF ALTERNATIVE CAPD FLUIDS - COMPARISON OF BICARBONATE-BUFFERED AND GLUCOSE-POLYMER-BASED SOLUTIONS", Nephrology, dialysis, transplantation, 9(7), 1994, pp. 785-790

Abstract

Evidence is accumulating that conventional dialysis fluids for CAPD are incompatible with peritoneal host defence. We therefore investigated the effect of alternative CAPD fluids on mononuclear leukocyte (PBMC) viability and cytokine production in vitro. Fluids tested were bicarbonate-buffered solutions containing 1.5% or 4.25% glucose, 7.5% glucose polymer dialysis fluid (GPDF), and conventional 1.5% glucose fluid (G1.5%). PBMC were stimulated (2 h, 37-degrees-C) in the different test fluids with a clinical isolate of Staphylococcus epidermidis or Escherichia coli lipopolysaccharide. The cytokines TNFalpha and IL-6 in PBMC supernatants - were measured by specific enzyme immunoassays. Induction of cytokine messenger RNA was evaluated by reverse transcription-polymerase chain reaction. Conventional G1.5% (pH 5.5) inhibited cytokine release from activated PBMC by >95%, whereas cell responses in low-glucose bicarbonate fluid were not significantly reduced. In contrast, high-glucose bicarbonate fluid exerted > 80% inhibition despite its neutral pH. GPDF was inhibitory at its initial low pH, whereas cytokine release was restored following pH neutralization. Cytokine mRNA expression was suppressed by conventional G1.5% fluid and by high-glucose bicarbonate fluid. These data indicate that pH neutralization leads toa substantial improvement of dialysis fluid biocompatibility; however, hyperosmolality and/or high glucose content inhibit cell responsiveness even at normal pH. Replacement of glucose by glucose polymer mightprove beneficial provided that the initial low pH is neutralized.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 05:32:25