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Titolo:
PSEUDOTHIONIN-ST1, A POTATO PEPTIDE ACTIVE AGAINST POTATO PATHOGENS
Autore:
MORENO M; SEGURA A; GARCIAOLMEDO F;
Indirizzi:
ETS INGN AGRONOMOS,BIOQUIM & BIOL MOLEC LAB E-28040 MADRID SPAIN ETS INGN AGRONOMOS,BIOQUIM & BIOL MOLEC LAB E-28040 MADRID SPAIN
Titolo Testata:
European journal of biochemistry
fascicolo: 1, volume: 223, anno: 1994,
pagine: 135 - 139
SICI:
0014-2956(1994)223:1<135:PAPPAA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT ANTIFUNGAL PROTEINS; NUCLEOTIDE-SEQUENCE; GAMMA-PUROTHIONINS; FAMILY; SEEDS; ENDOSPERM; CDNA; INHIBITORS; EXPRESSION; THIONINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M. Moreno et al., "PSEUDOTHIONIN-ST1, A POTATO PEPTIDE ACTIVE AGAINST POTATO PATHOGENS", European journal of biochemistry, 223(1), 1994, pp. 135-139

Abstract

A 5-kDa polypeptide, pseudothionin Solanum tuberosum 1(Pth-St1), which was active against Clavibacter michiganesis subspecies sepedonicus, a bacterial pathogen of potatoes, has been purified from the buffer-insoluble fraction of potato tubers by salt extraction and HPCL. Pth-St1was also active against other potato pathogens tested (Pseudomonas solanacearum and Fusarium solani). The N-terminal amino acid sequence ofthis peptide was identical (except for a N/H substitution at position2) to that deduced from a previously reported cDNA sequence (EMBL accession number X-13180), which had been misclassified as a Bowman-Birk protease inhibitor. Pth-St1 did not inhibit either trypsin or insect alpha-amylase activities, and, in contrast with true thionins, did not affect cell-free protein synthesis or beta-glucuronidase activity. Northern-blot and tissue-print analyses showed that steady-state mRNA levels were highest in flowers (especially in petals), followed by tubers(especially in the epidermal cell layers and in leaf primordia), stems and leaves. Infection of leaves with a bacterial pathogen suspended in 10 mM MgCl2 switched off the gene, whereas mock inoculation with 10mM MgCl2 alone induced higher mRNA levels.

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Documento generato il 19/09/20 alle ore 11:44:15