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Titolo:
PROENKEPHALIN TRANSGENIC MICE - A SHORT PROMOTER CONFERS HIGH TESTIS EXPRESSION AND REDUCED FERTILITY
Autore:
OHARA BF; DONOVAN DM; LINDBERG I; BRANNOCK MT; RICKER DD; MOFFATT CA; KLAUNBERG BA; SCHINDLER C; CHANG TSK; NELSON RJ; UHL GR;
Indirizzi:
STANFORD UNIV,DEPT BIOL SCI STANFORD CA 94305 NIDA,ADDICT RES CTR,MOLEC NEUROBIOL BRANCH BALTIMORE MD 21224 NIDA,ADDICT RES CTR,BEHAV PHARMACOL & GENET LAB BALTIMORE MD 21224 JOHNS HOPKINS UNIV,SCH MED,DEPT UROL BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT POPULAT DYNAM BALTIMORE MD 00000 JOHNS HOPKINS UNIV,SCH HYG BALTIMORE MD 00000 JOHNS HOPKINS UNIV,DEPT PSYCHOL BALTIMORE MD 21218 JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL BALTIMORE MD 21205 LOUISIANA STATE UNIV,SCH MED,DEPT BIOCHEM NEW ORLEANS LA 00000 LOUISIANA STATE UNIV,SCH MED,DEPT MOLEC BIOL NEW ORLEANS LA 00000
Titolo Testata:
Molecular reproduction and development
fascicolo: 3, volume: 38, anno: 1994,
pagine: 275 - 284
SICI:
1040-452X(1994)38:3<275:PTM-AS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTMEIOTIC GERM-CELLS; MESSENGER-RNA; GENE-EXPRESSION; CHLORAMPHENICOL ACETYLTRANSFERASE; REPRODUCTIVE-SYSTEM; SERTOLI CELLS; SOMATIC-CELLS; CYCLIC AMP; RAT TESTIS; TRANSCRIPTION;
Keywords:
ENKEPHALIN GENE; REGULATION; SPERMATOGENESIS; INFERTILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
B.F. Ohara et al., "PROENKEPHALIN TRANSGENIC MICE - A SHORT PROMOTER CONFERS HIGH TESTIS EXPRESSION AND REDUCED FERTILITY", Molecular reproduction and development, 38(3), 1994, pp. 275-284

Abstract

The regulation and possible function of the preproenkephalin gene in testis were studied in vivo in transgenic mice containing: (1) bases -193 to +210 of the human proenkephalin gene and an additional one kilobase of 3' proenkephalin flanking sequence driving expression of bacterial chloramphenicol acetyltransferase (CAT), and (2) the same promoter and flanking sequences driving expression of a rat proenkephalin cDNA. Five lines of mice, designated HEC1-5, expressed the first construct and 10, HER1-10, the second. Each HEC male and many HER males showeddramatic expression of the transgene in the testis, although much lower expression was observed in the brain and other enkephalin-producingtissues. High levels of expression in testis can thus be achieved with a very short promoter region and do not require intron A sequences previously considered necessary. Altered enkephalin expression may affect testicular function. One founder, HER8, displayed grossly abnormal testicular morphology and was completely infertile. A second founder, HER6, had low sperm motility. Two offspring from other lines also displayed subnormal fertility. These studies support a role for specific promoter sequences in testis expression and may further support a significant role for proenkephalin in testicular function. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:56:12