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Titolo:
REGULATION OF C-CADHERIN FUNCTION DURING ACTIVIN INDUCED MORPHOGENESIS OF XENOPUS ANIMAL CAPS
Autore:
BRIEHER WM; GUMBINER BM;
Indirizzi:
MEM SLOAN KETTERING CANC CTR,CELLULAR BIOCHEM & BIOPHYS PROGRAM,BOX 564,1275 YORK AVE NEW YORK NY 10021 MEM SLOAN KETTERING CANC CTR,CELLULAR BIOCHEM & BIOPHYS PROGRAM NEW YORK NY 10021 UNIV CALIF SAN FRANCISCO,PROGRAM BIOMED SCI SAN FRANCISCO CA 94143
Titolo Testata:
The Journal of cell biology
fascicolo: 2, volume: 126, anno: 1994,
pagine: 519 - 527
SICI:
0021-9525(1994)126:2<519:ROCFDA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CELL-ADHESION; MOLECULE E-CADHERIN; N-CADHERIN; TYROSINE PHOSPHORYLATION; MESODERM INDUCTION; BETA-CATENIN; EXPRESSION; EMBRYOS; LAEVIS; UVOMORULIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
W.M. Brieher e B.M. Gumbiner, "REGULATION OF C-CADHERIN FUNCTION DURING ACTIVIN INDUCED MORPHOGENESIS OF XENOPUS ANIMAL CAPS", The Journal of cell biology, 126(2), 1994, pp. 519-527

Abstract

Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this system to explore thepotential regulation of cell-cell adhesion and cadherin function during morphogenesis. Quantitative blastomere aggregation assays revealed that activininduction reduced the calcium-dependent adhesion between blastomeres. Activin-induced blastomeres formed smaller aggregates, anda greater proportion of the population remained as single cells compared to uninduced blastomeres. The aggregation was mediated by C-cadherin because C-cadherin was present in the blastomeres during the aggregation assay, and monoclonal antibodies against C-cadherin inhibited the calcium-dependent aggregation of blastomeres. E-cadherin was not detectable until after the completion of the assay and, therefore, does not explain the adhesive differences between induced and uninduced blastomeres. L cells stably expressing C-cadherin (LC cells) were used to demonstrate that C-cadherin activity was specifically altered after activin induction. Blastomeres induced with activin bound fewer LC cellsthan uninduced blastomeres. L cells not expressing C-cadherin did notadhere to blastomeres. The changes in C-cadherin-mediated adhesion occurred without detectable changes in the steady-state levels of C-cadherin or the amount of C-cadherin present on the surface of the cell. Immunoprecipitation of C-cadherin and its associated catenins revealed that the ratio of C-cadherin and the catenins was not altered by activin induction. These results demonstrate that activin decreases the adhesive function of existing C-cadherin molecules on the surface of blastomeres and suggest that decreased cadherin mediated cell-cell adhesion is associated with increased morphogenetic movement.

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Documento generato il 03/07/20 alle ore 01:28:42