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Titolo:
PROGLUCAGON PROCESSING IN PORCINE AND HUMAN PANCREAS
Autore:
HOLST JJ; BERSANI M; JOHNSEN AH; KOFOD H; HARTMANN B; ORSKOV C;
Indirizzi:
UNIV COPENHAGEN,PANUM INST,DEPT MED PHYSIOL,BLEGDAMSVEJ 3 DK-2200 COPENHAGEN N DENMARK NOVO NORDISK AS DK-2880 BAGSVAERD DENMARK UNIV COPENHAGEN,RIGSHOSP,DEPT CLIN BIOCHEM DK-2100 COPENHAGEN DENMARK
Titolo Testata:
The Journal of biological chemistry
fascicolo: 29, volume: 269, anno: 1994,
pagine: 18827 - 18833
SICI:
0021-9258(1994)269:29<18827:PPIPAH>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCAGON-LIKE PEPTIDE-1; HUMAN SMALL-INTESTINE; PIG SMALL-INTESTINE; PREPROGLUCAGON GENE; GLICENTIN; CONTAINS; SEQUENCE; PRODUCTS; GLP-1; FRAGMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Holst et al., "PROGLUCAGON PROCESSING IN PORCINE AND HUMAN PANCREAS", The Journal of biological chemistry, 269(29), 1994, pp. 18827-18833

Abstract

In the pancreas proglucagon (PG), a peptide precursor of 160 amino acids is cleaved to produce glucagon and a 30-amino acid N-terminal flanking peptide, but the fate of the C-terminal flanking peptide (99 amino acids) is incompletely known. We subjected acid ethanol extracts of human and porcine pancreases to gel filtration and analyzed the fractions with specific radioimmunoassays for the following regions of proglucagon: PG 62-69, PG 72-81, PG 78-87, PG 98-107 amide, PG 126-134, andPG 149-158. Based on these assays and successive purifications by high performance liquid chromatography we isolated and purified to homogeneity three porcine peptides which were subjected to mass spectrometryand sequencing. One peptide was PG 64-69. The second was PG 72-108, as determined by mass spectrometry, N-terminal amino acid sequencing, and specific radioimmunoassays. The third had a molecular size of approximately 10,000, an N-terminal sequence corresponding to PG 72-81, anda C terminal sequence terminating at PG 158 (specific radioimmunoassay). A similar peptide with an identical N-terminal sequence, a C-terminal sequence corresponding to PG 146-158, and a molecular mass of 9969(theoretical mass for human PG 72-158 = 9971) was isolated from humanpancreas together with small amounts of a peptide corresponding to PG72-107 amide. Thus, the pancreatic processing of the C-terminal flanking peptide in proglucagon includes the formation of equimolar (to glucagon) amounts of PG 64-69 and PG 72-158 (major proglucagon fragment) and smaller amounts of N-terminally extended glucagon-like peptide-1 (GLP-1) (PG 72-108 in pigs and PG 72-107 amide in humans).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 23:42:31