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Titolo:
ISOLATION AND CHARACTERIZATION OF TITIN T1 FROM BOVINE CARDIAC-MUSCLE
Autore:
PAN KM; DAMODARAN S; GREASER ML;
Indirizzi:
UNIV WISCONSIN,DEPT ANAT MADISON WI 53706 UNIV WISCONSIN,DEPT ANAT MADISON WI 53706 UNIV WISCONSIN,DEPT FOOD SCI MADISON WI 53706 UNIV WISCONSIN,MUSCLE BIOL LAB MADISON WI 53706
Titolo Testata:
Biochemistry
fascicolo: 27, volume: 33, anno: 1994,
pagine: 8255 - 8261
SICI:
0006-2960(1994)33:27<8255:IACOTT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED SKELETAL-MUSCLE; THICK FILAMENTS; ELASTIC PROTEIN; IMMUNOELECTRON MICROSCOPY; MYOFIBRILLAR PROTEINS; MONOCLONAL-ANTIBODIES; POLYACRYLAMIDE GELS; NATIVE CONNECTIN; ALPHA-CONNECTIN; MYOSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Pan et al., "ISOLATION AND CHARACTERIZATION OF TITIN T1 FROM BOVINE CARDIAC-MUSCLE", Biochemistry, 33(27), 1994, pp. 8255-8261

Abstract

Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts onSephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclonal antibodies against denatured titin stained the I band regions and weakly stained the M line area of cardiac myofibrils but primarily stained the A-I junction positionsin skeletal muscle myofibrils. Bovine cardiac titin contained 176 +/-5 mu mol of sulfhydryl groups per gram in the presence of 6 M guanidine hydrochloride or O.1% SDS; only 45% of these groups were reactive under non-denaturing condition. Circular dichroism measurements indicated that titin contained approximately 53% beta-sheet and 47% random coil at pH 7.0. These results are consistent with the idea that titin isprimarily composed of immunoglobulin C2 and fibronectin type III likemotifs throughout its length, including the flexible I band region.

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Documento generato il 07/07/20 alle ore 12:25:59