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Titolo:
LYSINE RESIDUES IN BEE VENOM PHOSPHOLIPASE A(2) ARE IMPORTANT FOR BINDING TO HUMAN MONOCLONAL OR POLYCLONAL ANTIBODIES OF THE IGG4 ISOTOPE
Autore:
SCHNEIDER T; DUDLER T; GELB MH; SUTER M;
Indirizzi:
SWISS INST ALLERGY & ASTHMA RES,OBERE STR 22 CH-7270 DAVOS SWITZERLAND UNIV WASHINGTON,DEPT CHEM SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 INST VIROL ZURICH SWITZERLAND
Titolo Testata:
International archives of allergy and immunology
fascicolo: 3, volume: 104, anno: 1994,
pagine: 262 - 269
SICI:
1018-2438(1994)104:3<262:LRIBVP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HONEYBEE VENOM; PROTEIN; IMMUNOTHERAPY; IMMUNIZATION; ALLERGY;
Keywords:
BEE VENOM PHOSPHOLIPASE A(2); DISCONTINUOUS B CELL EPITOPE; LYSINE RESIDUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
T. Schneider et al., "LYSINE RESIDUES IN BEE VENOM PHOSPHOLIPASE A(2) ARE IMPORTANT FOR BINDING TO HUMAN MONOCLONAL OR POLYCLONAL ANTIBODIES OF THE IGG4 ISOTOPE", International archives of allergy and immunology, 104(3), 1994, pp. 262-269

Abstract

Discontinuous antigenic sites on bee venom phospholipase A(2) (PLA) have been mapped using human monoclonal antibodies or human polyclonal serum antibodies (hpAbs) of the IgG4 isotype from beekeepers or of theIgE isotype from individuals allergic to PLA. Lysine residues of PLA have been specifically modified by acetylation or acylation by treatment with citraconic anhydride of their epsilon-amino groups to analyze their role in antigen-antibody binding. After the modifications, the binding of PLA to the human monoclonal antibodies is lost, whereas the binding to IgG4 hpAbs is significantly decreased. In contrast, the effect on the binding of PLA to IgE hpAbs appears to be more heterogeneous. The data indicate the importance of lysine residues as being part of B cell epitopes in PLA-specific antibodies of the IgG4 isotype, but less so for those of the IgE isotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:13:46