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Titolo:
SUPPRESSION OF ALLOANTIGEN-INDUCED T-CELL PROLIFERATION BY CD14(-COLONY-STIMULATING FACTOR-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS() CELLS DERIVED FROM GRANULOCYTE)
Autore:
MIELCAREK M; MARTIN PJ; TOROKSTORB B;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV CLIN RES,1124 COLUMBIA ST,M-318 SEATTLE WA 98104 UNIV WASHINGTON,DEPT MED SEATTLE WA 00000
Titolo Testata:
Blood
fascicolo: 5, volume: 89, anno: 1997,
pagine: 1629 - 1634
SICI:
0006-4971(1997)89:5<1629:SOATPB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARROW TRANSPLANTATION; PROGENITOR CELLS; LYMPHOCYTES-T; HOST DISEASE; STEM-CELLS; MACROPHAGES; INDUCTION; LYMPHOMA; MONOCYTES; LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M. Mielcarek et al., "SUPPRESSION OF ALLOANTIGEN-INDUCED T-CELL PROLIFERATION BY CD14(-COLONY-STIMULATING FACTOR-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS() CELLS DERIVED FROM GRANULOCYTE)", Blood, 89(5), 1997, pp. 1629-1634

Abstract

The proliferative responsiveness of granulocyte colony-stimulating factor (G-CSF)-mobilized blood was studied in uni-directional mixed leukocyte cultures. Unfractionated mononuclear cells from mobilized blood obtained by leukapheresis at day 4 after initiation of G-CSF (G-PBMC) were hyporesponsive (31.5%+/-9.2% response, P=.003) compared to mononuclear cells obtained from the peripheral blood before administration of G-CSF (preG-PBMC). There was great variability among donors when purified preG- and G-CD4 cells were compared, In eight of 10 donors, G-CD4 cells were equally responsive or moderately hyporesponsive; in two of 10 donors, G-CD4 cells were more strikingly hyporesponsive, CD14 cells derived from leukapheresis products (G-CD14 cells) suppressed alloantigen-induced proliferation by 48.6%+/-7.5% when added to preG-PBMC or preG-CD4 cells at responder-CD14 ratios of 2:1 (P <.001). Suppression was evident (14.4%+/-5.0%) even at responder-CD14 ratios of 8:1 and was largely contact-independent. PreG- and G-CD14 cells had equivalentpotency in suppressing proliferative responses. Given that G-CSF-mobilized blood cell grafts contain 50-fold more CD14 cells and only 10-fold more T cells than marrow, we propose that suppression of donor T cells by the large proportion of monocytes present in leukapheresis products could contribute to the unexpectedly low incidence and severity of graft-versus-host disease after peripheral blood stem cell transplantation. (C) 1997 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 01:22:36