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Titolo:
ALTERED HOST-RANGE PHENOTYPE OF THE TRANSFORMATION-DETECTIVE AD12 MUTANT CS-1 IS DUE TO DELETIONS IN THE E1 REGION
Autore:
OPALKA B; REITHWITOWSKI M; KIRCH HC; HOLTHAUSEN HS;
Indirizzi:
UNIV ESSEN GESAMTHSCH,SCH MED,INST MOLEC BIOL,W GERMAN CANC CTR,DEPT INTERNAL MED CANC RES D-45122 ESSEN GERMANY
Titolo Testata:
Intervirology
fascicolo: 1, volume: 37, anno: 1994,
pagine: 36 - 40
SICI:
0300-5526(1994)37:1<36:AHPOTT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ADENOVIRUS; MESSENGER-RNA; TUMOR-ANTIGEN; VERO CELLS; 5 DNA; PROTEIN; GENES;
Keywords:
ADENOVIRUS 12; HOST RANGE MUTANTS; E1 REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
B. Opalka et al., "ALTERED HOST-RANGE PHENOTYPE OF THE TRANSFORMATION-DETECTIVE AD12 MUTANT CS-1 IS DUE TO DELETIONS IN THE E1 REGION", Intervirology, 37(1), 1994, pp. 36-40

Abstract

Although it grows well in bulk infection, human adenovirus type 12 (Adl2) does not plaque efficiently in Vero cells of simian origin. Afterlong-term passage of the virus or after transfection of Ad12 DNA intothese cells, however, transformation-defective, host-range mutants giving high plaque yields in Vero cells were isolated. The original mutants have deletions in both Ela and Elb as well as additions of viral sequences at the right terminus of the genome. We have constructed a recombinant virus (Ad12d169) carrying both El alterations of the original mutant CS-1 on the Ad12 wild-type background. Another mutant (Ad12mut2) has additional sequences at the right terminus and an intact El region. In plaque assays mutant virus Ad12d169 carrying Ela deletions has an about thousandfold higher efficiency in Vero cells than Ad12wt and Ad12mut2, mapping the enhanced replication in Vero cells to the deletions in E1. Mechanical models for the influence of the E1 deletions, for example by up-regulation of the E2-encoded DNA-binding protein, are discussed to explain the efficient replication of mutant adenoviruses in Vero cells under plaque assay conditions.

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Documento generato il 01/11/20 alle ore 02:55:17