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Titolo:
SULFATE DETECTION IN GLYCOPROTEIN-DERIVED OLIGOSACCHARIDES BY ARTIFICIAL NEURAL-NETWORK ANALYSIS FOURIER-TRANSFORM INFRARED-SPECTRA
Autore:
POWELL DA; TURULA V; DEHASETH JA; VANHALBEEK H; MEYER B;
Indirizzi:
UNIV GEORGIA,COMPLEX CARBOHYDRATE RES CTR,220 RIVERBEND RD ATHENS GA 30602 UNIV GEORGIA,COMPLEX CARBOHYDRATE RES CTR ATHENS GA 30602 UNIV GEORGIA,DEPT BIOCHEM ATHENS GA 30602 UNIV GEORGIA,DEPT CHEM ATHENS GA 30602
Titolo Testata:
Analytical biochemistry
fascicolo: 1, volume: 220, anno: 1994,
pagine: 20 - 27
SICI:
0003-2697(1994)220:1<20:SDIGOB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPARAGINE-LINKED OLIGOSACCHARIDES; COMPLEX CARBOHYDRATE UNITS; PORCINE THYROGLOBULIN; CHONDROITIN SULFATE; STRUCTURAL-ANALYSIS; H-1-NMR SPECTRA; E-SELECTIN; GLYCOSAMINOGLYCANS; IDENTIFICATION; PROTEOGLYCANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Powell et al., "SULFATE DETECTION IN GLYCOPROTEIN-DERIVED OLIGOSACCHARIDES BY ARTIFICIAL NEURAL-NETWORK ANALYSIS FOURIER-TRANSFORM INFRARED-SPECTRA", Analytical biochemistry, 220(1), 1994, pp. 20-27

Abstract

We report the use of an artificial neural network to analyze the fingerprint region of Fourier-transform infrared (ir) spectra of oligosaccharides for the presence of sulfate groups. This assay can rapidly andnondestructively detect the presence of sulfate in as little as 1 nmol (approximate to 2 mu g) of a glycoprotein-derived monosulfated decasaccharide. The neural network was trained to recognize the presence ofsulfate groups by presenting it with 45 ir spectra of sulfated and nonsulfated mono- and oligosaccharides. No prior knowledge of the characteristic ir spectral features of a sulfate group was needed as input. The training process required between 3 and 10 h, while analysis of a spectrum with the trained neural network requires only 0.1 s. (C) 1994Academic Press,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:58:09