Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING THE POKEWEED ANTIVIRAL PROTEIN-II FROM PHYTOLACCA-AMERICANA AND ITS EXPRESSION IN ESCHERICHIA-COLI
Autore:
POYET JL; RADOM J; HOEVELER A;
Indirizzi:
UFR SCI & TECH,BIOCHIM & BIOL MOLEC LAB,16 ROUTE GRAY F-25030 BESANCON FRANCE UFR SCI & TECH,BIOCHIM & BIOL MOLEC LAB F-25030 BESANCON FRANCE
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 347, anno: 1994,
pagine: 268 - 272
SICI:
0014-5793(1994)347:2-3<268:IACOAC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; RIBOSOME-INACTIVATING PROTEINS; EUKARYOTIC RIBOSOMES; ESCHERICHIA-COLI; A CHAIN; SEQUENCE; PURIFICATION; IMMUNOTOXIN; RICIN; SITE;
Keywords:
CDNA; RIBOSOME-INACTIVATING PROTEIN; PHYTOLACCA AMERICANA; POKEWEED ANTIVIRAL PROTEIN FROM SUMMER LEAVES; EXPRESSION IN ESCHERICHIA-COLI; INHIBITION OF IN VITRO TRANSLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Poyet et al., "ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING THE POKEWEED ANTIVIRAL PROTEIN-II FROM PHYTOLACCA-AMERICANA AND ITS EXPRESSION IN ESCHERICHIA-COLI", FEBS letters, 347(2-3), 1994, pp. 268-272

Abstract

Three distinct ribosome-inactivating proteins (RIPs) were isolated from pokeweed (Phytolacca americana). We identified and sequenced for the first time a complete cDNA encoding the pokeweed antiviral protein II (PAP II), which is expressed in the late summer leaves of pokeweed. The cDNA of PAP II consists of 1,187 nucleotides and encodes a mature protein of 285 amino acids. Its predicted amino acid sequence is only 33% similar to PAP and PAP-S. The NH2 terminal extrapeptide (25 amino acid residues) was similar but not identical to that of PAP's extrapeptide. The cDNA of PAP II was expressed in E. coli. The growth of the transformants was strongly inhibited after induction of the gene. Furthermore, PAP II, which was produced in E. coli, inhibited protein synthesis in a rabbit reticulocyte translation system. Thus, recombinant PAP II would appear to be as functional as native PAP in inhibiting protein synthesis in both prokaryotes and eukaryotes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:01:03