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Titolo:
ISOLATION AND CHARACTERIZATION OF THE CHICKEN M2 ACETYLCHOLINE-RECEPTOR PROMOTER REGION - INDUCTION OF GENE-TRANSCRIPTION BY LEUKEMIA INHIBITORY FACTOR AND CILIARY NEUROTROPHIC FACTOR
Autore:
ROSOFF ML; WEI J; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,BOX 357750 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 25, volume: 93, anno: 1996,
pagine: 14889 - 14894
SICI:
0027-8424(1996)93:25<14889:IACOTC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCARINIC RECEPTOR; MESSENGER-RNAS; CELL-LINE; EXPRESSION; NEURONS; PROTEIN; CLONING; HEART; BRAIN; SUBTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Rosoff et al., "ISOLATION AND CHARACTERIZATION OF THE CHICKEN M2 ACETYLCHOLINE-RECEPTOR PROMOTER REGION - INDUCTION OF GENE-TRANSCRIPTION BY LEUKEMIA INHIBITORY FACTOR AND CILIARY NEUROTROPHIC FACTOR", Proceedings of the National Academy of Sciences of the United Statesof America, 93(25), 1996, pp. 14889-14894

Abstract

We have isolated the promoter region and determined the start sites of transcription for the gene encoding the chicken m2 (cm2) muscarinic acetylcholine receptor. Transfection experiments, using cm2-luciferasereporter gene constructs, demonstrated that a 789-bp genomic fragment,vas sufficient to drive high level expression in chicken heart primary cultures, while an additional 1,2-kb region was required for maximalexpression in mouse septal/neuroblastoma (SN56) cells, Treatment of SN56 cells with the cytokines ciliary neurotrophic factor and leukemia inhibitory factor increases expression of endogenous muscarinic acetylcholine receptors and results in a 4- to 6-fold induction of cm2 promoter driven luciferase expression. We have mapped a region of the cm2 promoter that is necessary for induction by cytokines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:37:48