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Titolo:
INTERFERON-GAMMA-INDUCED EXPRESSION OF MHC ANTIGENS FACILITATES IDENTIFICATION OF DONOR CELLS IN CHIMERIC TRANSPLANT RECIPIENTS
Autore:
RAO AS; DEMETRIS AJ; QIAN SG; MURASE N; LI YP; FUNG JJ; STARZL TE;
Indirizzi:
UNIV PITTSBURGH,MED CTR,DEPT SURG,3601 5TH AVE,5C FALK CLIN PITTSBURGH PA 15213 UNIV PITTSBURGH,MED CTR,DEPT SURG PITTSBURGH PA 15213 UNIV PITTSBURGH,MED CTR,PITTSBURGH TRANSPLANT INST PITTSBURGH PA 15213 UNIV PITTSBURGH,MED CTR,DEPT PATHOL PITTSBURGH PA 15213
Titolo Testata:
Cell transplantation
fascicolo: 4, volume: 3, anno: 1994,
pagine: 345 - 348
SICI:
0963-6897(1994)3:4<345:IEOMAF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; CLASS-II; ALLOTRANSPLANTATION; INDUCTION; RATS;
Keywords:
INTERFERON-GAMMA; CHIMERISM; MHC CLASS I AND CLASS II; RODENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Rao et al., "INTERFERON-GAMMA-INDUCED EXPRESSION OF MHC ANTIGENS FACILITATES IDENTIFICATION OF DONOR CELLS IN CHIMERIC TRANSPLANT RECIPIENTS", Cell transplantation, 3(4), 1994, pp. 345-348

Abstract

After whole organ transplantation, donor bone marrow-derived cells migrate out of the graft into the recipient, leading to establishment ofchimerism, which is the first step towards the subsequent induction of donor-specific tolerance. In routine immunohistochemical staining, monoclonal antibodies specific for heterotopic MHC alleles are used to identify donor and recipient cells. However, it is difficult to detectthese cells using this technique in longterm allograft recipients whohave a persistently low donor cell population (microchimerism). Because Interferon-gamma (IFN-gamma) is known to induce expression of MHC class I and class II cell surface molecules, we used this cytokine 12-48 h before sacrifice, to facilitate the identification of donor and recipient cells in the tissues of animals transplanted with either liver(B10 --> C3H) or bone marrow (LEW --> BN). In long-term allograft recipients, the use of IFN-gamma for as briefly as 12 h prior to sacrifice, results in marked upregulation of class I and class II antigens, leading to easy identification of ubiquitously distributed low numbers of donor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 15:25:47