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Titolo:
INVESTIGATION OF THE STR LOCUS HUMTH01 USING PCR AND 2 ELECTROPHORESIS FORMATS - UK AND GALICIAN CAUCASIAN POPULATION SURVEYS AND USEFULNESS IN PATERNITY INVESTIGATIONS
Autore:
LAREU MV; PHILLIPS CP; CARRACEDO A; LINCOLN PJ; COURT DS; THOMSON JA;
Indirizzi:
UNIV SANTIAGO DE COMPOSTELA,DEPT LEGAL MED SANTIAGO SPAIN UNIV LONDON LONDON HOSP,COLL MED,DEPT HAEMATOL LONDON E1 ENGLAND
Titolo Testata:
Forensic science international
fascicolo: 1, volume: 66, anno: 1994,
pagine: 41 - 52
SICI:
0379-0738(1994)66:1<41:IOTSLH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; TANDEM REPEAT LOCI; AMPLIFICATION; DNA;
Keywords:
STR; HUMTHO1; POPULATION; PATERNITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M.V. Lareu et al., "INVESTIGATION OF THE STR LOCUS HUMTH01 USING PCR AND 2 ELECTROPHORESIS FORMATS - UK AND GALICIAN CAUCASIAN POPULATION SURVEYS AND USEFULNESS IN PATERNITY INVESTIGATIONS", Forensic science international, 66(1), 1994, pp. 41-52

Abstract

Two hundred unrelated Caucasians from the UK and 210 from Galicia (NWSpain) have been genotyped for the HUMTH (0) over cap 1 locus using polymerase chain reaction (PCR) amplification followed by high sieving agarose electrophoresis (UK samples) and polyacrylamide electrophoresis (Galician samples). Allele and genotype frequencies obtained from both populations were in close agreement to those seen by other workers and chi(2) tests of the two populations showed that both were in Hardy-Weinberg equilibrium. The potential usefulness of HUMTH01 in paternity investigations was analysed by constructing, within each population,false family trios, each with a non-father, in order to estimate the actual exclusion rate. The observed rate of exclusion was in close agreement with the expected rate for both populations. Examination of themother-child pairs in the false families showed a common allele in every case and no evidence of mutation or non-Mendelian inheritance was observed. The HUMTH01 locus shows an informative polymorphism and the production and analysis of population databases is easily achieved. The use of PCR followed by electrophoresis in either gel format appears to provide a quick and straightforward method for investigating the HUMTH01 locus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:03:24