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Titolo:
DETECTION OF FLAGELLAR ANTIGEN OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI IN CANINE FECES WITH AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) - NEW PROSPECTS FOR DIAGNOSIS
Autore:
MONFORT JD; BECHNIESEN JD; STILLS HF;
Indirizzi:
OHIO STATE UNIV,COLL VET MED,DEPT VET PREVENT MED COLUMBUS OH 43210
Titolo Testata:
Veterinary research communications
fascicolo: 2, volume: 18, anno: 1994,
pagine: 85 - 92
SICI:
0165-7380(1994)18:2<85:DOFAOC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Keywords:
ANTIGEN; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; DOG; DIAGNOSIS; ENZYME-LINKED IMMUNOASSAY; FECES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Monfort et al., "DETECTION OF FLAGELLAR ANTIGEN OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI IN CANINE FECES WITH AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) - NEW PROSPECTS FOR DIAGNOSIS", Veterinary research communications, 18(2), 1994, pp. 85-92

Abstract

A new diagnostic procedure was developed to detect the flagellar antigen of Campylobacter jejuni and Campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. Extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluatedusing species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. The assay detected all C. jejuni or C. coli infected specimens compared with direct selective faecal culture. One of 18 faecal specimens culture-negative for C. jejuni was identified as positive by the assay, i.e. a false positive rate of 1 of 18 (5.6%) and a corresponding specificity of 94.4%. These results suggest that the screening procedure developed to detect flagellar antigens of C. jejuni and C, coli in canine faecal samples should be further investigated as a diagnostic alternative to culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 19:57:16