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Titolo:
INTERACTION BETWEEN THE FIRST AND LAST NUCLEOTIDES OF PRE-MESSENGER-RNA INTRONS IS A DETERMINANT OF 3' SPLICE-SITE SELECTION IN SACCHAROMYCES-CEREVISIAE
Autore:
CHANFREAU G; LEGRAIN P; DUJON B; JACQUIER A;
Indirizzi:
INST PASTEUR,DEPT BIOL MOLEC,CNRS,URA 1149,UNITE GENET MOLEC LEVURES,25 RUE DR ROUX F-75724 PARIS 15 FRANCE INST PASTEUR,DEPT BIOL MOLEC,CNRS,URA 1149,UNITE GENET MOLEC LEVURES F-75724 PARIS 15 FRANCE
Titolo Testata:
Nucleic acids research
fascicolo: 11, volume: 22, anno: 1994,
pagine: 1981 - 1987
SICI:
0305-1048(1994)22:11<1981:IBTFAL>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; ACTIVE-SITE; U6 SNRNA; U1 SNRNA; YEAST; GENE; MUTATIONS; SEQUENCES; MUTANTS; LARIAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
G. Chanfreau et al., "INTERACTION BETWEEN THE FIRST AND LAST NUCLEOTIDES OF PRE-MESSENGER-RNA INTRONS IS A DETERMINANT OF 3' SPLICE-SITE SELECTION IN SACCHAROMYCES-CEREVISIAE", Nucleic acids research, 22(11), 1994, pp. 1981-1987

Abstract

The splicing of group II and nuclear pre-mRNAs introns occurs via a similar splicing pathway and some of the RNA-RNA interactions involved in these splicing reactions show structural similarities. Recently, genetic analyses performed in a group II intron and the yeast nuclear actin gene suggested that non Watson-Crick interactions between intron boundaries are important for the second splicing step efficiency in both classes of introns. We here show that, in the yeast nuclear rp51A intron, a G to ($) under bar A mutation at the first position activates cryptic 3' splice sites with the sequences UA ($) under bar C/ or UA ($) under bar A/. Moreover, the natural 3' splice site could be reactivated by a G to ($) under bar C substitution of the last intron nucleotide. These results demonstrate that the interaction between the first and last intron nucleotides is a conserved feature of nuclear pre-mRNAsplicing in yeast and is involved in the mechanism of 3' splice site selection.

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Documento generato il 04/12/20 alle ore 12:34:29