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Titolo:
DETECTION OF PROLIFERATING CELL NUCLEAR ANTIGEN ANALOG IN 4 SPECIES OF MARINE-PHYTOPLANKTON
Autore:
LIN SJ; CHANG J; CARPENTER EJ;
Indirizzi:
SUNY STONY BROOK,MARINE SCI RES CTR STONY BROOK NY 11794
Titolo Testata:
Journal of phycology
fascicolo: 3, volume: 30, anno: 1994,
pagine: 449 - 456
SICI:
0022-3646(1994)30:3<449:DOPCNA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA POLYMERASE-DELTA; AUXILIARY PROTEIN; GROWTH-RATES; PCNA CYCLIN; S-PHASE; REPLICATION; GENE; EXPRESSION; POPULATIONS; SEQUENCE;
Keywords:
CELL CYCLE PROTEIN; DUNALIELLA TERTIOLECTA; GROWTH RATE; ISOCHRYSIS GALBANA; PCNA; PHYTOPLANKTON CELL CYCLE; PROLIFERATING CELL NUCLEAR ANTIGEN; SKELETONEMA COSTATUM; THALASSIOSIRA WEISSFLOGII; WESTERN BLOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Lin et al., "DETECTION OF PROLIFERATING CELL NUCLEAR ANTIGEN ANALOG IN 4 SPECIES OF MARINE-PHYTOPLANKTON", Journal of phycology, 30(3), 1994, pp. 449-456

Abstract

Proliferating cell nuclear antigen (PCNA) is an auxiliary protein forpolymerase-delta and therefore is essential for cellular DNA synthesis. The synthesis and abundance of pcna in the cell are cell-cycle-dependent, both increasing markedly during the S phase. Such a protein could be a useful cell cycle marker, which is required for estimaing algal species-specific growth rates via the cell cycle approach. By using commercially available monoclonal antirat-PCNA antibody and an enhanced chemiluminescence technique, PCNA-like proteins were detected in four species of marine phytoplankton. The strong single band detected on western blots of Isochrysis galbana Parke, Thalassiosira weissflogii Cleve, and Dunaliella tertiolecta butcher had an apparent molecular weight of 33-36 kda. This molecular weight is within the range as observed for PCNA in a wide phylogenetic array of organisms (33-36 kDa). In the diatom Skeletonema costatum (Grev. ) Cleve, the PCNA antibody detected a major band of about 19 kDa as well as a minor band of 38 kDa. Thedetected proteins were specifically recognized by the monoclonal anti-rat-PCNA antibody. The PCNA-like proteins in I. galbana, T. weissflogii, and D. tertiolecta were more abundant in the exponential growth stage and then decreased and became undetectable in the late stationary stage. Our results show that the detected antigens appear to be algal analogs of PCNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:41:32