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Titolo:
COORDINATE EXPRESSION AND PROLIFERATIVE ROLE OF HOXB GENES IN ACTIVATED ADULT T-LYMPHOCYTES
Autore:
CARE A; TESTA U; BASSANI A; TRITARELLI E; MONTESORO E; SAMOGGIA P; CIANETTI L; PESCHLE C;
Indirizzi:
THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,BLUEMLE LIFE SCI BLDG,ROOM 528,233 S 10TH ST PHILADELPHIA PA 19107 THOMAS JEFFERSON UNIV,JEFFERSON CANC INST PHILADELPHIA PA 19107 IST SUPER SANITA,DEPT HEMATOL & ONCOL I-00161 ROME ITALY CNR,IST MED SPERIMENTALE I-00137 ROME ITALY
Titolo Testata:
Molecular and cellular biology
fascicolo: 7, volume: 14, anno: 1994,
pagine: 4872 - 4877
SICI:
0270-7306(1994)14:7<4872:CEAPRO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONAL CARCINOMA-CELLS; HUMAN HEMATOPOIETIC-CELLS; CENTRAL-NERVOUS-SYSTEM; HOMEOBOX GENE; RETINOIC ACID; LEUKEMIA; PROGENITORS; DIFFERENTIATION; TRANSLOCATION; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Care et al., "COORDINATE EXPRESSION AND PROLIFERATIVE ROLE OF HOXB GENES IN ACTIVATED ADULT T-LYMPHOCYTES", Molecular and cellular biology, 14(7), 1994, pp. 4872-4877

Abstract

We investigated the expression of HOXB cluster genes in purified phytohemagglutinin (PHA)-activated T lymphocytes from normal adult peripheral blood by reverse transcription PCR and RNase protection. These genes are not expressed in quiescent T cells, except for barely detectable BI RNA. After the PHA stimulus, HOXB gene activation initiates coordinately as a rapid induction wave in the 3'--> 5' cluster direction (i.e., from HOXB1 through B9 genes). Thus, (i) expression of the foremost S'-located BI and B2 genes peaks 10 min after PKA addition and then rapidly declines, (ii) activation of B3, B4, and B5 begins 10 min after PHA addition and peaks at later times i.e., at 120 min for B5), (iii) B6, B7, and B9 are expressed at a low level starting at later times (45 to 60 min), and (iv) B8 remains silent. Treatment of PHA-activatedT lymphocytes with antisense oligonucleotides to B2 or B4 mRNA causesa drastic inhibition of T-cell proliferation and a decreased expression of T-cell activation markers (i.e., interleukin 2 and transferrin receptors). Similarly, treatment of CEM-CCRF, Peer, and SEZ627 T acute lymphocytic leukemia cell lines with anti-B4 oligomer markedly inhibits cell proliferation. Finally, T cells stimulated by a low dosage of PHA in the presence of 1 mu M retinoic acid show a marked increase of both HOXB expression, particularly B2, and cell proliferation. These studies provide novel evidence on the role of HOX genes in adult cell proliferation. (i) Coordinate, early activation of HOXB genes from the 3'--> 5' cluster side apparently underlies T-cell activation. (ii) The expression pattern in adult PHA-activated T cells is strikingly similar to that observed in retinoic acid-induced teratocarcinoma cells (A. Simeone, D. Acampora, L. Arcioni, P. W. Andres, E. Boncinelli, and F. Mavilio, Nature (London) 346:763-766, 1990), thus suggesting that molecular mechanisms underlying HOX gene expression in the earliest stagesof development may also operate in activated adult T lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:08:18