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Titolo:
THE HOMOLOGY OF A NOVEL POLYPEPTIDE WITH STRESS PROTEIN-CHARACTERISTICS IN EMBRYONIC MICE BRAIN AND IN THE HYPOTHALAMUS OF CALORIC RESTRICTED RATS AS DETERMINED BY ULTRAMICRO WESTERN BLOTTING
Autore:
PIPKIN JL; HINSON WG; LYNCOOK LE; LABORDE J; ANSON JF; FEUERS RJ; DUFFY PH; HART RW; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DEPT HLTH & HUMAN SERV,DIV GENET TOXICOL,HFT-120 JEFFERSON AR 72079
Titolo Testata:
Electrophoresis
fascicolo: 5, volume: 15, anno: 1994,
pagine: 677 - 684
SICI:
0173-0835(1994)15:5<677:THOANP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYACRYLAMIDE-GEL ELECTROPHORESIS; SODIUM DODECYL-SULFATE; HEAT-SHOCK PROTEINS; CELL-CYCLE; NUCLEAR-PROTEIN; LIMITED PROTEOLYSIS; HL-60 CELLS; INDUCTION; PHOSPHORYLATION; ARSENITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Pipkin et al., "THE HOMOLOGY OF A NOVEL POLYPEPTIDE WITH STRESS PROTEIN-CHARACTERISTICS IN EMBRYONIC MICE BRAIN AND IN THE HYPOTHALAMUS OF CALORIC RESTRICTED RATS AS DETERMINED BY ULTRAMICRO WESTERN BLOTTING", Electrophoresis, 15(5), 1994, pp. 677-684

Abstract

A novel protein (p34) was observed in polyacrylamide gel fluorographsof gestation day 13 embryonic mouse brain following retinoic acid dosing of dams. Another p34 polypeptide with identical gel migratory characteristics was seen in the hypothalamus of old caloric restricted rats after ''food deprivation stress''. Western blotting, employing an ultramicro trans-blot cell developed in our laboratory, detected identical immunochemical determinants between these proteins, verifying theirhomology. Peptide mapping and Western blotting further validated the uniqueness of p34 compared with other stress proteins including heme oxygenase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:28:41