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Titolo:
TREHALOSE - A CRYOPROTECTANT THAT ENHANCES RECOVERY AND PRESERVES FUNCTION OF HUMAN PANCREATIC-ISLETS AFTER LONG-TERM STORAGE
Autore:
BEATTIE GM; CROWE JH; LOPEZ AD; CIRULLI V; RICORDI C; HAYEK A;
Indirizzi:
UNIV CALIF SAN DIEGO,SCH MED,WHITTIER INST DIABET & ENDOCRINOL,DEPT PEDIAT,9894 GENESEE AVE LA JOLLA CA 92037 UNIV CALIF SAN DIEGO,SCH MED,WHITTIER INST DIABET & ENDOCRINOL,DEPT PEDIAT LA JOLLA CA 92037 UNIV CALIF DAVIS,SECT MOL & CELLULAR BIOL DAVIS CA 95616 UNIV MIAMI,DIABET RES INST MIAMI FL 33152
Titolo Testata:
Diabetes
fascicolo: 3, volume: 46, anno: 1997,
pagine: 519 - 523
SICI:
0012-1797(1997)46:3<519:T-ACTE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FETAL PANCREAS; NEWLY DEVELOPED SOLUTIONS; CANINE LUNG PRESERVATION; PHASE-TRANSITIONS; CRYOPRESERVATION; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
G.M. Beattie et al., "TREHALOSE - A CRYOPROTECTANT THAT ENHANCES RECOVERY AND PRESERVES FUNCTION OF HUMAN PANCREATIC-ISLETS AFTER LONG-TERM STORAGE", Diabetes, 46(3), 1997, pp. 519-523

Abstract

The scarcity of available tissue for transplantation in diabetes and the need for multiple donors make it mandatory to use an optimal cryopreservation method that allows maximal recovery and preservation of beta-cell function, We have developed a method to cryopreserve islets with excellent survival of endocrine cells, Current methods use DMSO as cryoprotectant. Our method involves introducing both DMSO and the disaccharide trehalose into the cells during cooling. Uptake and release of trehalose occurred during the thermotropic lipid-phase transition measured in pancreatic endocrine cells between 5 degrees and 9 degrees C, using [C-14]trehalose, Recovery of adult islets after cryopreservation with 300 mmol/l trehalose was 92 vs. 58% using DMSO alone, In vitrofunction, in terms of insulin content and release in response to secretagogues, was indistinguishable from fresh islets, Grafts from isletscryopreserved with trehalose contained 14-fold more insulin than grafts from islets cryopreserved without trehalose, Results with human fetal islet-like cell clusters (ICCs) were more pronounced: recovery fromcryopreservation was 94%, compared with 42% without trehalose, Complete functionality of fetal cells was also restored; tritiated thymidineincorporation and insulin content and release were similar to fresh tissue, After transplantation in nude mice, there was a 15-fold increase in insulin content of grafts from ICCs cryopreserved with trehalose compared with ICCs cryopreserved without trehalose, Thus, the additionof trehalose to cryopreservation protocols leads to previously unobtainable survival rates of human pancreatic endocrine tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 20:22:31