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Titolo:
DIFFERENTIAL-EFFECTS OF GONADOTROPIN AND ORTHOVANADATE ON OOCYTE MATURATION, OVULATION, AND PROSTAGLANDIN SYNTHESIS BY RANA OVARIAN FOLLICLES IN-VITRO
Autore:
CHANG KJ; KIM JW; IM WB; KANG HM; KWON HB;
Indirizzi:
CHONNAM NATL UNIV,DEPT BIOL KWANGJU 500757 SOUTH KOREA CHONNAM NATL UNIV,DEPT BIOL KWANGJU 500757 SOUTH KOREA CHONNAM NATL UNIV,HORMONE RES CTR KWANGJU 500757 SOUTH KOREA CHONGJU UNIV,DEPT GENET ENGN CHONGJU 360764 SOUTH KOREA
Titolo Testata:
The Journal of experimental zoology
fascicolo: 2, volume: 277, anno: 1997,
pagine: 155 - 165
SICI:
0022-104X(1997)277:2<155:DOGAOO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT SALVELINUS-FONTINALIS; PROGESTERONE PRODUCTION; INVOLVEMENT; INVITRO; INDUCTION; PROTEIN; CAMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K.J. Chang et al., "DIFFERENTIAL-EFFECTS OF GONADOTROPIN AND ORTHOVANADATE ON OOCYTE MATURATION, OVULATION, AND PROSTAGLANDIN SYNTHESIS BY RANA OVARIAN FOLLICLES IN-VITRO", The Journal of experimental zoology, 277(2), 1997, pp. 155-165

Abstract

Effects of gonadotropin and sodium orthovanadate on oocyte maturation, ovulation, prostaglandin, and progesterone synthesis were examined during in vitro culture of Rana ovarian follicles obtained from hibernating animals. Frog pituitary homogenates (FPH, 0.05 gland/ml) effectively induced oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation (>90%) in ovarian follicles obtained in mid-hibernation (mid-December through late January). In contrast, orthovanadate induced a limited amount (<45%) of ovulation of some oocytes without concomitant induction of maturation during mid-hibernation. In late hibernation (February to early March), considerable spontaneous maturation and ovulation occurred in cultured ovarian follicles. During this period both FPH (0.0005-0.05 gland/ml) or orthovanadate (0.01-1 mM) treatment markedly increased the incidence of ovulation and accelerated the onset of ovulation in a dose dependent manner. When examined after 12 h of culture, few (<5%) oocytes ovulated in response to orthovanadate had undergone GVBD whereas most (>90%) of those ovulated in response to FPH hadmatured. However, the majority of oocytes ovulated (72%) within 6 h of exposure to FPH had intact GVs, indicating that GVBD is also not a prerequisite for ovulation induction in response to FPH. Orthovanadate stimulated PGF(2 alpha) in a dose dependent manner but failed to stimulate progesterone production whereas FPH stimulated secretion of both PGF(2 alpha) and progesterone. The amount and time course of PGF(2 alpha) secretion in response to orthovanadate were similar to results produced with FPH stimulation. Treatment of PGF(2 alpha) to follicles obtained in late hibernation also accelerated the ovulation of the oocytes. Taken together, the data suggest that orthovanadate enhanced ovulation of immature oocytes was mediated via enhanced PGF(2 alpha) production and that oocyte maturation is not essential or prerequisite for invitro oocyte ovulation in Rana. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:19:03