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Titolo:
THE ISOLATION AND PARTIAL CHARACTERIZATION OF ALPHA(1)-PROTEINASE INHIBITOR FROM THE SERUM OF THE OSTRICH (STRUTHIO-CAMELUS)
Autore:
KUHN CR; NAUDE RJ; TRAVIS J; OELOFSEN W;
Indirizzi:
UNIV PORT ELIZABETH,DEPT BIOCHEM,POB 1600 PORT ELIZABETH 6000 SOUTH AFRICA UNIV PORT ELIZABETH,DEPT BIOCHEM PORT ELIZABETH 6000 SOUTH AFRICA UNIV GEORGIA,DEPT BIOCHEM ATHENS GA 30602
Titolo Testata:
International Journal of Biochemistry
fascicolo: 6, volume: 26, anno: 1994,
pagine: 843 - 853
SICI:
0020-711X(1994)26:6<843:TIAPCO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA ALPHA-1-PROTEINASE INHIBITOR; PROTEINASE-INHIBITORS; ALPHA-1 ANTITRYPSIN; MULTIPLE FORMS; ALPHA-1-ANTITRYPSIN; PANCREAS; ALPHA-1-ANTICHYMOTRYPSIN; CHYMOTRYPSINOGEN; PURIFICATION; INACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Kuhn et al., "THE ISOLATION AND PARTIAL CHARACTERIZATION OF ALPHA(1)-PROTEINASE INHIBITOR FROM THE SERUM OF THE OSTRICH (STRUTHIO-CAMELUS)", International Journal of Biochemistry, 26(6), 1994, pp. 843-853

Abstract

1. Native and cleaved alpha(1)-proteinase inhibitor was purified fromostrich serum using Sepharose-blue dextran chromatography, ammonium sulfate precipitation and ion exchange chromatography on DEAE-Toyopearl650 M at pH 8.8 and 6.5.2. Ostrich alpha(1)-PI displayed M(r) values of 68,100 using gradient PAGE and 66,200 using Ferguson plots. 3. Isoelectric focusing of ostrich alpha(1)-PI in the pH range 3-10 revealed pi values of 4.84 and 4.91, and in the pH range 4-6 the characteristicmicroheterogeneity observed for mammalian alpha(1)-PIs was displayed.4. The presence of sialic acid, hexoses and hexosamines was detected using chemical methods, but were found in much lower quantities as compared to alpha(1)-PIs of other species. 5. Western blot analysis demonstrated a positive reaction between the native and cleaved ostrich alpha(1)-PIs and the antibodies to the ostrich alpha(1)-PIs raised in rabbits. No cross-reactivity was demonstrated by Western blot analysis between human alpha(1)-PI and antibodies to ostrich alpha(1)-PI. 6. The inhibitory effect of alpha(1)-PI on elastase and chymotrypsin was alsoinvestigated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:41:12