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Titolo:
ATP GATED POTASSIUM CHANNELS IN ACUTE MYOCARDIAL HIBERNATION AND REPERFUSION
Autore:
OFFSTAD J; KIRKEBOEN KA; ILEBEKK A; DOWNING SE;
Indirizzi:
ULLEVAL HOSP,INST EXPTL MED RES N-0407 OSLO NORWAY YALE UNIV,SCH MED,DEPT PATHOL NEW HAVEN CT 06510
Titolo Testata:
Cardiovascular Research
fascicolo: 6, volume: 28, anno: 1994,
pagine: 872 - 880
SICI:
0008-6363(1994)28:6<872:AGPCIA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE K+ CHANNELS; PERFUSED RAT HEARTS; CONTRACTILE FAILURE; CARDIAC-FUNCTION; INFARCT SIZE; ISCHEMIA; DOGS; CROMAKALIM; NICORANDIL; MECHANISM;
Keywords:
ADENOSINE RECEPTORS; APRIKALIM; CORONARY FLOW; GLIBENCLAMIDE; ISOLATED HEART; LOW FLOW ISCHEMIA; PIGLET; RABBIT; REPERFUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J. Offstad et al., "ATP GATED POTASSIUM CHANNELS IN ACUTE MYOCARDIAL HIBERNATION AND REPERFUSION", Cardiovascular Research, 28(6), 1994, pp. 872-880

Abstract

Objective: ATP gated potassium (K-ATP) channels and adenosine are of crucial importance in coronary blood flow regulation and activation ofK-ATP channels and adenosine receptor stimulation protect against infarction and development of stunning. The aim of this study was to testthe hypothesis that opening of K-ATP channels and adenosine receptor stimulation are involved in perfusion-contraction matching, in acute hibernation, and in recovery after reperfusion. Methods: 30 isolated piglet hearts (2-10 d old) and 20 isolated rabbit hearts were studied. The isolated piglet hearts were perfused with modified Krebs Henseleit (KH) solution enriched by washed human red blood cells; the isolated rabbit hearts were perfused with modified KH buffer. The effects of theK-ATP channel opener aprikalim (1 mu M), the K-ATP channel antagonistglibenclamide (30 mu M), and the adenosine receptor antagonist 8-(p-sulphophenyl)theophylline (SPT, 300 mu M) on 2 h of low flow (10%) ischaemia and 1 h reperfusion were compared with saline in the piglet hearts. The effects of aprikalim (1 mu M), glibenclamide (30 mu M), and saline during 90 min of low flow (10%) ischaemia followed by 1 h reperfusion were also examined in the isolated rabbit hearts. Results: At constant coronary flow aprikalim reduced perfusion pressure from 53(SEM 5) to 25(1) mm Hg (p<0.001) in piglet hearts and from 55(5) to 39(5) mmHg (p<0.05) in rabbit hearts. Glibenclamide increased perfusion pressure from 47(5) to 61(6) mm Hg (p<0.01) in piglet hearts and from 45(4)to 81(5) mm Hg (p<0.001) in rabbit hearts. SPT increased perfusion pressure from 55(6) to 67(6) mm Hg (p<0.05) in piglet hearts. Left ventricular systolic pressure remained unchanged in both models. During stepwise reductions in coronary flow a parallel stepwise reduction in left ventricular systolic pressure was observed in all groups. At 2 h of low flow ischaemic systolic pressure was 39(4)%, 37(5)%, 41(4)% and 37(3)% of control for hearts treated with saline aprikalim, glibenclamide, and SPT, respectively. During the low flow period systolic pressureand MV(O2) stabilised. An almost identical pattern occurred in rabbithearts. After 30 min of recovery of piglet hearts left ventricular systolic pressure increased to 78(5)% (saline), 74(5)% (aprikalim), 84(5)% (glibenclamide), and 77(4)% (SPT) of control. The recovery as percentage of control in rabbit hearts was 63(11) (saline), 69(8) (aprikalim) and 56(13) adenosine receptor blockade. K-ATP channels do not modulate either perfuion-contraction matching or acute hibernation and functional recovery during reperfusion in the red blood cell perfused piglet heart or the crystalloid perfused rabbit hearts. Moreover, adenosine receptor antagonism does not affect these phenomena in piglet hearts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:16:46