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Titolo:
CONTRIBUTION OF INDIVIDUAL BASE-PAIRS TO THE INTERACTION OF TFIIIA WITH THE XENOPUS 5S RNA GENE
Autore:
VELDHOEN N; YOU QM; SETZER DR; ROMANIUK PJ;
Indirizzi:
UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,POB 3055 VICTORIA V8W 3P6 BC CANADA UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL VICTORIA V8W 3P6 BC CANADA CASE WESTERN RESERVE UNIV,SCH MED,DEPT MOLEC BIOL & MICROBIOL CLEVELAND OH 44106
Titolo Testata:
Biochemistry
fascicolo: 24, volume: 33, anno: 1994,
pagine: 7568 - 7575
SICI:
0006-2960(1994)33:24<7568:COIBTT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR-IIIA; INTERNAL CONTROL REGION; DIRECTS SPECIFIC INITIATION; FINGER DNA RECOGNITION; ZINC-BINDING DOMAINS; CRYSTAL-STRUCTURE; B-FORM; FUNCTIONAL DOMAINS; LAEVIS OOCYTES; 5S-RNA GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
N. Veldhoen et al., "CONTRIBUTION OF INDIVIDUAL BASE-PAIRS TO THE INTERACTION OF TFIIIA WITH THE XENOPUS 5S RNA GENE", Biochemistry, 33(24), 1994, pp. 7568-7575

Abstract

The effects of a series of point mutations within the Xenopus borealis somatic-type 5S RNA gene on transcription factor IIIA (TFIIIA) binding affinity were quantified. These data define a critical sequence-dependent contact region within the classical box C promoter element frombase pair 80 to 91. Substitution of GC base pairs at positions 81, 85, 86, 89, and 91 significantly reduce TFIIIA binding affinity. Base pairs located at other positions within the box C contact region providea moderate contribution to TFIIIA-5S gene interaction. In contrast tothe extensive set of sequence contacts within the box C element, TFIIIA interaction is localized primarily to two GC base pairs at positions 70 and 71 within the intermediate promoter element. A selected amplification and binding assay (SAAB) was performed with a synthetic internal control region (ICR) randomized from base pair 78 to 95 to identify box C promoter sequences bound with high affinity by TFIIIA. The wild-type 5S RNA gene sequence from 79 to 92 is strongly selected. These results are consistent with the critical role of the box C element in sequence-dependent promoter recognition by TFIIIA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:35:34