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Titolo:
ACTIVATION OF NOVEL PROTEIN-KINASES C-DELTA AND C-EPSILON UPON MITOGENIC STIMULATION OF QUIESCENT RAT 3Y1 FIBROBLASTS
Autore:
OHNO S; MIZUNO K; ADACHI Y; HATA A; AKITA Y; AKIMOTO K; OSADA S; HIRAI S; SUZUKI K;
Indirizzi:
YOKOHAMA CITY UNIV,SCH MED,DEPT MOLEC BIOL,KANAZAWA KU,3-9 FUKU URA YOKOHAMA 236 JAPAN TOKYO METROPOLITAN INST MED SCI,DEPT MOLEC BIOL,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,INST MOLEC & CELLULAR BIOSCI,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 26, volume: 269, anno: 1994,
pagine: 17495 - 17501
SICI:
0021-9258(1994)269:26<17495:AONPCA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROTROPIN-RELEASING-HORMONE; DIFFERENTIAL DOWN-REGULATION; 2 DISTINCT CLASSES; PHORBOL ESTER; NPKC-EPSILON; GH4C1 CELLS; 3T3 CELLS; PHOSPHORYLATION; EXPRESSION; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
S. Ohno et al., "ACTIVATION OF NOVEL PROTEIN-KINASES C-DELTA AND C-EPSILON UPON MITOGENIC STIMULATION OF QUIESCENT RAT 3Y1 FIBROBLASTS", The Journal of biological chemistry, 269(26), 1994, pp. 17495-17501

Abstract

Rat fibroblast 3Y1 cells express at least three protein kinase C species, conventional PKC alpha (cPKC alpha), novel PKC delta (nPKC delta), and novel PKC epsilon (nPKC epsilon). The stimula tion of quiescent 3Y1 cells by serum (or epidermal growth factor (EGF)) but not 12-O-tetradecanoylphorbol-13-acetate (TPA) results in the induction of DNA synthesis. Upon stimulation by serum or EGF, endogenous PKC species showed no indication of activation such as translocation or down-regulation, whereas TPA or synthetic diacylglycerol caused activation of all these PKC species when judged by these criteria. The only indication of activation observed upon serum or EGF stimulation was an upward shift in the electrophoretic mobility of nPKC delta. The phosphorylation levels of endogenous PKC members determined by in vivo metabolic labeling experiments revealed increased phosphorylation of both nPKC delta and nPKC epsilon, but only a slight increase for cPKC alpha in response toserum or EGF. On the other hand, TPA caused increased phosphorylationof all three PKC species. Overexpression of these PKC members by introduction of the corresponding cDNA expression plasmids resulted in theenhancement of the cell response to TPA when monitored in terms of transcriptional activation through TPA- or serum-responsive elements. Such enhancement in transcriptional activation by overexpression of cPKCalpha, nPKC delta, or nPKC epsilon was also observed in response to diacylglycerol, indicating that all these PKC species are activated by diacylglycerol in cells. In contrast to these nonphysiological stimuli, serum (or EGF) stimulation of 3Y1 cells that overexpress the respective PKC members revealed a clear difference between cPKC and nPKC, in that overexpression of nPKC delta or nPKC epsilon resulted in a large increase in TPA- or serum-responsive element activation, whereas the overexpression of cPKC alpha increased activation only very slightly. These results indicate that the mitogenic stimulation of quiescent 3Y1 cells results in selective activation of endogenous nPKC members and that the modes of activation of cPKC and nPKC differ from each other.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:11:16