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Titolo:
FUNCTIONAL DISSECTION OF THE YEAST CYC8-TUP1 TRANSCRIPTIONAL CO-REPRESSOR COMPLEX
Autore:
TZAMARIAS D; STRUHL K;
Indirizzi:
HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,240 LONGWOOD AVE BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL BOSTON MA 02115
Titolo Testata:
Nature
fascicolo: 6483, volume: 369, anno: 1994,
pagine: 758 - 761
SICI:
0028-0836(1994)369:6483<758:FDOTYC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING SPECIFICITY; SACCHAROMYCES-CEREVISIAE; GLUCOCORTICOID RECEPTOR; GLUCOSE REPRESSION; ACTIVATION DOMAIN; ESCHERICHIA-COLI; SHUTTLE VECTORS; PROTEIN; GENES; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D. Tzamarias e K. Struhl, "FUNCTIONAL DISSECTION OF THE YEAST CYC8-TUP1 TRANSCRIPTIONAL CO-REPRESSOR COMPLEX", Nature, 369(6483), 1994, pp. 758-761

Abstract

DNA-BINDING repressor proteins mediate regulation of yeast genes by cell type (Mcm1/alpha 2 and a1/alpha 2), glucose (Mig1) and oxygen (Rox1) (refs 1-4 respectively). An unusual feature of all these regulatorypathways is that transcriptional repression requires two physically associated proteins(5) that do not bind DNA Cyc8(Ssn6) and Tup1. The Cyc8-Tup1 complex has been proposed to be a corepressor that is recruited to target promoters by pathway-specific DNA-binding proteins(6), butthe specific functions of the individual proteins are unknown. Here we show that when it is bound upstream of a functional promoter throughthe LexA DNA-binding domain, Tup1 represses transcription in the absence of Cyc8. Deletion analysis indicates that Tup1 contains at least two non-overlapping transcriptional repression regions with minimal primary sequence similarity, and a separable Cyc8-interaction domain. These Tup1 domains, which do not include the beta-transducin motifs(7), are necessary and partially sufficient for Tup1 function. We suggest that Tup1 performs the repression function of the Cyc8-Tup1 co-repressorcomplex, and that Cyc8 serves as a link with the pathway-specific DNA-binding proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 04:19:22