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Titolo:
SURFACE-AREA CYCLING OF DIFFERENT SURFACTANT PREPARATIONS - SP-A AND SP-B ARE ESSENTIAL FOR LARGE-AGGREGATE INTEGRITY
Autore:
VELDHUIZEN RAW; HEARN SA; LEWIS JF; POSSMAYER F;
Indirizzi:
ST JOSEPHS HLTH CTR,LAWSON RES INST,DEPT MED,ROOM 4-504,268 GROSVENORST LONDON N6A 4V2 ON CANADA UNIV WESTERN ONTARIO,DEPT MED LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT BIOCHEM LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT PATHOL LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,MRC,FETAL & NEONATAL HLTH & DEV GRP LONDON N6A 5A5 ON CANADA
Titolo Testata:
Biochemical journal
, volume: 300, anno: 1994,
parte:, 2
pagine: 519 - 524
SICI:
0264-6021(1994)300:<519:SCODSP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS SYNDROME; LIPID EXTRACT SURFACTANT; PULMONARY SURFACTANT; CHEMICAL-COMPOSITION; BIOPHYSICAL ACTIVITY; ALVEOLAR SURFACTANT; LUNG SURFACTANT; TUBULAR MYELIN; PROTEIN; ADSORPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
R.A.W. Veldhuizen et al., "SURFACE-AREA CYCLING OF DIFFERENT SURFACTANT PREPARATIONS - SP-A AND SP-B ARE ESSENTIAL FOR LARGE-AGGREGATE INTEGRITY", Biochemical journal, 300, 1994, pp. 519-524

Abstract

Surface-area cycling is an in vitro procedure for the conversion of large into small surfactant aggregates. In this procedure a tube containing a surfactant suspension is rotated end-over-end at 37 degrees C so that the surface area of the suspension changes twice each cycle. Wehave utilized this method to study the mechanisms involved in aggregate conversion. Several different surfactant preparations were analysed: (1) bovine natural surfactant, a sucrose-gradient-purified material containing surfactant phospholipid and surfactant-associated proteins (SP-) SP-A, SP-B and SP-C; (2) bovine lipid-extract surfactant, which contains the surfactant phospholipids and SP-B and SP-C; (3) mixtures of dipalmitoyl phosphatidylcholine and phosphatidylglycerol (7:3, w/w)reconstituted with one or more surfactant proteins. Aggregate conversion was measured by phosphorus analysis of a 40000 g supernatant (small aggregate) and pellet (large aggregates) before and after surface-area cycling. Surface-area cycling of lipid extract surfactant or lipidsplus SP-B or SP-C resulted in rapid aggregate conversion. Lipids alone were not converted. Only a small percentage of purified natural surfactant was converted into small aggregates. Addition of SPA to lipid extract surfactant could inhibit aggregate conversion of this material,but this was only observed when an additional 1% (w/w) of SP-B was added to the lipid extract. It is concluded that SP-A is important for large-aggregate integrity. It appears that SP-A acts in conjunction with SP-B. The presence of SP-B and/or SP-C is required for aggregate conversion; it is proposed that this reflects the necessity for lipid adsorption in aggregate conversion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:20:24