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Titolo:
MONITORING TRICYCLIC ANTIDEPRESSANT CONCENTRATIONS IN SERUM BY FLUORESCENCE POLARIZATION IMMUNOASSAY COMPARED WITH GAS-CHROMATOGRAPHY AND HPLC
Autore:
RAO ML; STABEROCK U; BAUMANN P; HIEMKE C; DEISTER A; CUENDET C; AMEY M; HARTTER S; KRAEMER M;
Indirizzi:
UNIV BONN,PSYCHIAT KLIN & POLIKLIN,SIGMUND FREUD STR 25 D-53105 BONN GERMANY UNIV LAUSANNE,DEPT PSYCHIAT ADULTE,UNITE BIOCHIM CH-1008 LAUSANNE SWITZERLAND UNIV LAUSANNE,DEPT PSYCHIAT ADULTE,PSYCHOPHARMACOL CLIN CH-1008 LAUSANNE SWITZERLAND UNIV MAINZ,PSYCHIAT KLIN D-55101 MAINZ GERMANY
Titolo Testata:
Clinical chemistry
fascicolo: 6, volume: 40, anno: 1994,
pagine: 929 - 933
SICI:
0009-9147(1994)40:6<929:MTACIS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; ENZYME-IMMUNOASSAY; SPECIFICITY DATA; AMITRIPTYLINE; NORTRIPTYLINE; DRUGS; ASSAY; METHODOLOGY; PLASMA;
Keywords:
TOXICOLOGY; KIT ADAPTATION; COMPLIANCE; INTERMETHOD COMPARISON; NEUROACTIVE DRUGS; MONITORING THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Rao et al., "MONITORING TRICYCLIC ANTIDEPRESSANT CONCENTRATIONS IN SERUM BY FLUORESCENCE POLARIZATION IMMUNOASSAY COMPARED WITH GAS-CHROMATOGRAPHY AND HPLC", Clinical chemistry, 40(6), 1994, pp. 929-933

Abstract

The fluorescence polarization immunoassay (FPIA) developed by Abbott to diagnose intoxication with tricyclic antidepressants was adapted for therapeutic drug monitoring and validated with chromatographic methods to investigate its potential for this use. We compared serum concentrations of tricyclic antidepressants in vivo and in vitro obtained byFPIA with those by gas chromatography and HPLC. For amitriptyline, imipramine, clomipramine, and doxepin, the detection limit of the FPIA was 72, 71, 64, and 72 nmol/L (similar to 20 mu g/l), respectively; that by gas chromatography was 18, 18, and 16 nmol/L (similar to 5 mu g/L) for amitriptyline, imipramine and clomipramine, respectively; with HPLC the lower limit of detection for doxepin was 36 nmol/L (10 mu g/L), The intra- and interassay CVs ranged from 3% to 6%. In patients being treated with amitriptyline, imipramine, clomipramine, and doxepin, at steady-state the correlation coefficients between FPIA and GC/HPLC results for split samples were 0.95, 0.92, 0.90 and 0.70, respectively. However, the slopes were close to unity only for amitriptyline and doxepin, being 0.6 for imipramine and 1.9 for clomipramine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:36:33