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Titolo:
USE OF TRPE FUSION PROTEIN TO IDENTIFY ANTIGENIC DOMAINS WITHIN THE BIV ENVELOPE PROTEIN
Autore:
CHEN P; LIU ZQ; WOOD C;
Indirizzi:
UNIV KANSAS,DEPT MICROBIOL LAWRENCE KS 66045 UNIV KANSAS,DEPT MICROBIOL LAWRENCE KS 66045
Titolo Testata:
Journal of virological methods
fascicolo: 3, volume: 47, anno: 1994,
pagine: 331 - 343
SICI:
0166-0934(1994)47:3<331:UOTFPT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-LIKE VIRUS; MULTIPLE RETROVIRAL INFECTIONS; BOVINE LEUKEMIA-VIRUS; WESTERN-BLOT ASSAY; ESCHERICHIA-COLI; RECOMBINANT BACULOVIRUS; NUCLEOTIDE-SEQUENCE; CORE PROTEIN; CATTLE; GENE;
Keywords:
BOVINE IMMUNODEFICIENCY-LIKE VIRUS; ENVELOPE PROTEIN; TRPE; FUSION PROTEIN; (ESCHERICHIA-COLI);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P. Chen et al., "USE OF TRPE FUSION PROTEIN TO IDENTIFY ANTIGENIC DOMAINS WITHIN THE BIV ENVELOPE PROTEIN", Journal of virological methods, 47(3), 1994, pp. 331-343

Abstract

Nine different recombinant clones spanning various regions of the bovine immunodeficiency-like virus (BIV) envelope gene open reading framewere generated. These clones span the entire external glycoprotein aswell as the transmembrane glycoprotein region. These proteins were expressed as fusions to the TrpE protein in E. coli. The levels of recombinant protein expressed varied, some clones expressed enough protein that can be detected in a Coomassie blue-stained gel, whereas other proteins could only be detected by Western blot analyses. A recombinant env protein representing the extracellular domain of the env protein was detected by BIV-infected bovine sera. In addition, a 134 amino acidpeptide which may represent a major immunoreactive epitope was identified. This peptide is located at the amino terminus of the transmembrane glycoprotein and was specifically recognized by all BIV-infected calf sera tested. The identification of this epitope and the use of recombinant envelope protein will enable us to develop a more effective screening test to study the epidemiology of BIV infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:58:20