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Titolo:
THE ROLE OF THE EXTRACELLULAR SHEATH IN RECOGNITION AND ATTACHMENT OFCONIDIA OF DISCULA-UMBRINELLA (BERK-AND-BR) MORELET TO THE HOST SURFACE
Autore:
VIRET O; TOTI L; CHAPELA IH; PETRINI O;
Indirizzi:
STN FED RECH AGRON CHANGINS CH-1260 NYON SWITZERLAND ETH ZENTRUM,INST MICROBIOL CH-8092 ZURICH SWITZERLAND
Titolo Testata:
New phytologist
fascicolo: 1, volume: 127, anno: 1994,
pagine: 123 - 131
SICI:
0028-646X(1994)127:1<123:TROTES>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOXYLON-FRAGIFORME; ENDOPHYTIC SYMBIOSIS; ERYSIPHE-GRAMINIS; INFECTION COURT; LECTIN BINDING; FUNGUS; SPECIFICITY; ADHESION; CUTINASE; ESTABLISHMENT;
Keywords:
EXTRACELLULAR MATRIX; ADHESION; DISCULA UMBRINELLA; FAGUS SYLVATICA (BEECH); ENDOPHYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
O. Viret et al., "THE ROLE OF THE EXTRACELLULAR SHEATH IN RECOGNITION AND ATTACHMENT OFCONIDIA OF DISCULA-UMBRINELLA (BERK-AND-BR) MORELET TO THE HOST SURFACE", New phytologist, 127(1), 1994, pp. 123-131

Abstract

The role of the conidial sheath in the recognition process and in theattachment of conidia of the beech endophyte, Discula umbrinella, to the host surface was investigated. After treatment of conidia with different lectins the adhesion of conidia to the host surface was effectively inhibited. A strong fluorescence was observed after treatment of conidia with TRITC-labelled concanavalin A. Fluorescence with TRITC-labelled wheat germ agglutinin was observed only on spores subjected to enzymatic digestion with proteases. Observations with the transmissionelectron microscope (TEM), after labelling sections with colloidal gold conjugated lectins, confirmed the presence of mannose and/or glucose in the extracellular sheath. Chitin was present in the conidial cellwall but not in the extracellular sheath. Enzymatic treatment of the conidial sheath resulted in strongly reduced attachment and changes inthe binding of fluorescence-labelled lectins. TEM studies of partially digested conidia revealed that snail enzyme modified only slightly the structure of the sheath, while proteases completely dissolved the fibrillar sheath leaving a comparatively smooth cell wall with no fibrillar structures on its surface. We conclude that a proteinaceous sheath is responsible for the adhesion of Discula umbrinella conidia to thehost and that glycoproteins are involved in the recognition and attachment process. This attachment mechanism closely parallels the only other recognition system studied biochemically in endophytes, and is similar to the few analogous cases studied in plant-pathogenic fungi, pointing to the functional relatedness of endophytic and pathogenic interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:44:17