Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FRUCTAN SYNTHESIS IN OAT .1. OLIGOMER ACCUMULATION IN STEMS DURING COLD HARDENING AND THEIR IN-VITRO SYNTHESIS IN A CRUDE ENZYME EXTRACT
Autore:
LIVINGSTON DP; KNIEVEL DP; GILDOW FE;
Indirizzi:
PENN STATE UNIV,USDA ARS,US REG PASTURE RES LAB UNIV PK PA 16802 PENN STATE UNIV,DEPT AGRON UNIV PK PA 16802 PENN STATE UNIV,DEPT PLANT PATHOL UNIV PK PA 16802
Titolo Testata:
New phytologist
fascicolo: 1, volume: 127, anno: 1994,
pagine: 27 - 36
SICI:
0028-646X(1994)127:1<27:FSIO.O>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOLIUM-TEMULENTUM L; L CV GERBEL; EXCISED LEAVES; SUCROSE FRUCTOSYLTRANSFERASE; RIGIDUM GAUDIN; METABOLISM; INVERTASE; BIOSYNTHESIS; CARBOHYDRATE; HYDROLASE;
Keywords:
AVENA SATIVA (OAT); FRUCTAN OLIGOMERS; 1-KESTOSE; NEOKESTOSE; 6-KESTOSE; NYSTOSE; FRUCTOSYLTRANSFERASE ENZYMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
D.P. Livingston et al., "FRUCTAN SYNTHESIS IN OAT .1. OLIGOMER ACCUMULATION IN STEMS DURING COLD HARDENING AND THEIR IN-VITRO SYNTHESIS IN A CRUDE ENZYME EXTRACT", New phytologist, 127(1), 1994, pp. 27-36

Abstract

The accumulation of fructan oligomers in cold hardened oat stems was compared to oligomer synthesis in a crude enzyme extract. Water-soluble carbohydrates were quantified at specified time intervals in three oat cultivars which had been hardened for a total of 5 wk at 2 degrees C. A crude enzyme extract was prepared from stems of one cultivar hardened for 6 d at 2 degrees C and incubated at 30 degrees C with 200 mM sucrose. 1-Kestose was the first oligomer to accumulate in vitro but neokestose rapidly increased after 3 h of incubation and eventually surpassed the level of 1-kestose. Neokestose and 1-kestose accumulated atsimilar rates in vivo in the first week of hardening but after 1 week, 1-kestose began to decrease while neokestose remained relatively constant. The same phenomenon was observed in vitro but after only 3 h. 6-Kestose was not observed in vivo or in vitro, except for a trace before hardening began. The first tetramer to increase was 1&6(G)-kestotetraose but it subsequently decreased and its concentration was surpassed by that of 6(G),6-kestotetraose. Similarities and differences between in vivo and in vitro patterns of fructan synthesis are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 19:14:21