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Titolo:
SPATIAL-DISTRIBUTION OF 2 MATERNAL MESSENGERS IN PARACENTROTUS-LIVIDUS DURING OOGENESIS AND EMBRYOGENESIS
Autore:
DICARLO M; ROMANCINO DP; MONTANA G; GHERSI G;
Indirizzi:
CNR,IST BIOL SVILUPPO I-90123 PALERMO ITALY DIPARTIMENTO BIOL CELLULARE & SVILUPPO I-90123 PALERMO ITALY
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 12, volume: 91, anno: 1994,
pagine: 5622 - 5626
SICI:
0027-8424(1994)91:12<5622:SO2MMI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EMBRYO; DROSOPHILA EMBRYO; STRONGYLOCENTROTUS-PURPURATUS; XENOPUS OOCYTES; TOLL GENE; EXPRESSION; PROTEIN; POLARITY; PATTERN; RNA;
Keywords:
SEA URCHIN; IN SITU HYBRIDIZATION; MESSENGER-RNA LOCALIZATION; CELL SURFACE PROTEINS; PROTEIN LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M. Dicarlo et al., "SPATIAL-DISTRIBUTION OF 2 MATERNAL MESSENGERS IN PARACENTROTUS-LIVIDUS DURING OOGENESIS AND EMBRYOGENESIS", Proceedings of the National Academy of Sciences of the United Statesof America, 91(12), 1994, pp. 5622-5626

Abstract

We demonstrated that two mRNAs that are synthesized during the vitellogenic period of oogenesis and that code for cell surface proteins areasymmetrically distributed in the unfertilized egg of Paracentrotus lividus. At fertilization, these RNAs rapidly localize in the cortical zone at the animal pole of the egg. They are then detected in the mesomeres and the macromeres, but not in the micromeres, and thereafter are found in the ectoderm but not in the vegetal plate, mesenchyme cells, or early intestine. They disappear in late gastrula. The proteins synthesized by these mRNAs show the same territorial location during theperiod examined here, which included the unfertilized egg and the 16-blastomere stage. These conclusions were reached on the basis of in situ hybridization and immunostaining experiments, as web as Northern and Western blot analyses of isolated blastomeres. The possible significance of this asymmetric distribution of these two mRNAs and proteins in the establishment of the animal/vegetal axis is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:34:30