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Titolo:
EFFECTS OF HORMONES AND CYSTEINE PROTEASE MODULATORS ON INFECTION OF HEPG2 CELLS BY PLASMODIUM-BERGHEI SPOROZOITES IN-VITRO DETERMINED BY ELISA IMMUNOASSAY
Autore:
KUMAR S; VANPELT JF; ODOWD CA; HOLLINGDALE MR; SINDEN RE;
Indirizzi:
UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOL,MOLEC & CELLULAR PARASITOL GRP LONDON SW7 2BB ENGLAND
Titolo Testata:
The Journal of parasitology
fascicolo: 3, volume: 80, anno: 1994,
pagine: 414 - 420
SICI:
0022-3395(1994)80:3<414:EOHACP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOERYTHROCYTIC STAGES; MONOCLONAL-ANTIBODY; MALARIA PARASITES; LIVER-STAGE; INVITRO; HEPATOCYTES; FALCIPARUM; INTERFERON; ANTIGEN; PROBE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S. Kumar et al., "EFFECTS OF HORMONES AND CYSTEINE PROTEASE MODULATORS ON INFECTION OF HEPG2 CELLS BY PLASMODIUM-BERGHEI SPOROZOITES IN-VITRO DETERMINED BY ELISA IMMUNOASSAY", The Journal of parasitology, 80(3), 1994, pp. 414-420

Abstract

An enzyme-linked imunosorbent assay (ELISA) detecting a Plasmodium berghei liver-stage-specific protein Pbl-1 is described. The quantitative detection limits ranged from 0.01 to 0.05 mu g of parasite protein. Qualitatively the assay detected as little as 0.001 mu g Pbl-1 per well. Using the ELISA dexamethasone and insulin together was shown to promote higher parasite infections in HepG2 cells compared to unsupplemented medium. Anti-cowpea-protease cysteine inhibitor significantly increased hepatocyte invasion as compared to controls, whereas a significant decrease was recorded in the presence of the protease inhibitor E64. Partial involvement of cysteine proteases in HepG2 invasion by P. berghei sporozoites is therefore suggested.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:05:28