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Titolo:
MAPPING OF UPSTREAM REGULATORY ELEMENTS IN THE P4 PROMOTER OF PARVOVIRUS MINUTE VIRUS OF MICE
Autore:
FAISST S; PERROS M; DELEU L; SPRUYT N; ROMMELAERE J;
Indirizzi:
DEUTSCH KREBSFORSCHUNGSZENTRUM,APPL TUMOR VIROL PROGRAM,ABT 0610,POSTFACH 101949 D-69009 HEIDELBERG GERMANY INSERM,U375 D-69009 HEIDELBERG GERMANY INST PASTEUR,MOLEC ONCOL UNIT,CNRS,URA 1160 F-59019 LILLE FRANCE
Titolo Testata:
Virology
fascicolo: 1, volume: 202, anno: 1994,
pagine: 466 - 470
SICI:
0042-6822(1994)202:1<466:MOUREI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMED RAT-CELLS; AUTONOMOUS PARVOVIRUS; TRANSCRIPTION FACTOR; SENSITIZATION; RESOLUTION; SEQUENCE;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
S. Faisst et al., "MAPPING OF UPSTREAM REGULATORY ELEMENTS IN THE P4 PROMOTER OF PARVOVIRUS MINUTE VIRUS OF MICE", Virology, 202(1), 1994, pp. 466-470

Abstract

The early promoter (P4) of the autonomous parvovirus minute virus of mice (prototype strain) directs the expression of the transcription unit coding for the nonstructural proteins NS1 and NS2. Although proximal promoter elements (GC and TATA boxes) are essential for P4 activity in vivo, additional upstream sequences appear to be required for optimal transcription. Therefore, associations of proteins with the upstream regulatory region of promoter P4 were studied in the rat fibroblast cell line FRE14 by gel retardation and in vitro as well as in vivo footprinting assays. This led to the identification of at least four distinct upstream elements that interacted with cellular proteins. The functionality of these elements was supported by the reduced level of gene expression driven by corresponding linker-substitutive mutants of promoter P4. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:05:10