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Titolo:
COMPARISON OF RIBOSOMAL DNA LENGTH AND RESTRICTION SITE POLYMORPHISMSIN GREMMENIELLA AND ASCOCALYX ISOLATES
Autore:
BERNIER L; HAMELIN RC; OUELLETTE GB;
Indirizzi:
UNIV LAVAL,FAC FORESTERIE & GEOMAT,CTR RECH BIOL FORESTIERE,CITE UNIVQUEBEC CITY G1K 7P4 QUEBEC CANADA LAURENTIAN FORESTRY CTR ST FOY G1V 4C7 PQ CANADA
Titolo Testata:
Applied and environmental microbiology
fascicolo: 4, volume: 60, anno: 1994,
pagine: 1279 - 1286
SICI:
0099-2240(1994)60:4<1279:CORDLA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA GENE; ABIETINA; IDENTIFICATION; AMPLIFICATION; CHARACTERS; MARKERS; INTRON; FUNGI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
L. Bernier et al., "COMPARISON OF RIBOSOMAL DNA LENGTH AND RESTRICTION SITE POLYMORPHISMSIN GREMMENIELLA AND ASCOCALYX ISOLATES", Applied and environmental microbiology, 60(4), 1994, pp. 1279-1286

Abstract

The small subunit (SSU) and the internal transcribed spacer (ITS) of nuclear ribosomal DNA genes from 27 specimens of the fungal genera Gremmeniella and Ascocalyx were amplified by PCR. Length polymorphisms were observed in the SSU and allowed the differentiation of four groups among the isolates tested: (i) Ascocalyx abietis; (ii) Gremmeniella isolates from Picea spp.; (iii) Gremmeniella isolates from Abies balsamea; and (iv) Gremmeniella isolates from Abies sacchalinensis, Larix spp., and Pinus spp. The amplified ITS was the same length for all Gremmeniella specimens and was 60 bp longer in A. abietis. Phylogenetic analysis of length polymorphisms and of 24 restriction sites in the SSU and ITS showed that Gremmeniella isolates were more related to each other than to the Ascocalyx isolate. Furthermore, seven groups were evident within the genus Gremmeniella. Our results confirm that Gremmeniellaand Ascocalyx should be kept as different taxa and suggest that the taxonomy of the former could be revised to consider isolates from Abiesbalsamea and from Picea spp. to be two different varieties while incorporating Gremmeniella laricina into G. abietina, as a new variety.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:14:41