Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLE OF CELL-VOLUME VARIATIONS IN NA-K+-ATPASE RECRUITMENT AND()OR ACTIVATION IN CORTICAL COLLECTING DUCT/
Autore:
COUTRY N; FARMAN N; BONVALET JP; BLOTCHABAUD M;
Indirizzi:
UER XAVIER BICHAT,INSERM,U246,16 RUE HENRI HUCHARD F-75877 PARIS 18 FRANCE
Titolo Testata:
The American journal of physiology
fascicolo: 5, volume: 266, anno: 1994,
parte:, 1
pagine: 30001342 - 30001349
SICI:
0002-9513(1994)266:5<30001342:ROCVIN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOGEN-SYNTHESIS; ION-TRANSPORT; SODIUM; NEPHRON; PERMEABILITY; STIMULATION; EXPRESSION; MECHANISM; EPITHELIA; TURNOVER;
Keywords:
CELL SODIUM; MINERALOCORTICOIDS; OUABAIN BINDING; RUBIDIUM UPTAKE; MOUSE KIDNEY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
N. Coutry et al., "ROLE OF CELL-VOLUME VARIATIONS IN NA-K+-ATPASE RECRUITMENT AND()OR ACTIVATION IN CORTICAL COLLECTING DUCT/", The American journal of physiology, 266(5), 1994, pp. 30001342-30001349

Abstract

The aim of this study was to examine whether cell volume variations could play a role in the previously reported Na+-K+-ATPase pump recruitment and/or activation induced by an increase in intracellular Na concentration (Nail in cortical collecting ducts (CCD). Isolated CCD from kidneys of aldosterone-repleted mice were incubated in hyper-, hypo-, or isosmotic solutions with and without Na to modify Nai and cell volume independently. Nai, cell volume, and the number of basolateral pumps were measured using Na-22, image analysis, and specific [H-3]ouabainbinding, respectively. Ouabain-sensitive Rb-86 uptake was also measured. In CCD with high Nai, pump recruitment and/or activation was observed only when an increase in tubular volume was associated with Na load. Pump recruitment and/or activation was also induced by cell swelling in the absence of Na load. Recruited and/or activated pumps display an affinity for ouabain and a specific activity (ouabain-sensitive Rb uptake per pump unit) similar to basal pumps. We conclude that 1) cellswelling is implied in the process of Nai-dependent pump recruitment and/or activation, 2) cell swelling can promote pump recruitment and/or activation independently of Na load, 3) basal and recruited and/or activated pumps probably correspond to the same Na+-K+-ATPase isoform.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:57:05