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Titolo:
EFFECTS OF TEMPERATURE AND FREEZING ON HEPATOCYTES ISOLATED FROM A FREEZE-TOLERANT FROG
Autore:
STOREY KB; MOMMSEN TP;
Indirizzi:
CARLETON UNIV,DEPT BIOL,1125 COLONEL BY DR OTTAWA K1S 5B6 ON CANADA CARLETON UNIV,INST BIOCHEM OTTAWA K1S 5B6 ON CANADA
Titolo Testata:
The American journal of physiology
fascicolo: 5, volume: 266, anno: 1994,
parte:, 2
pagine: 180001477 - 180001482
SICI:
0002-9513(1994)266:5<180001477:EOTAFO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
WOOD FROG; RANA-SYLVATICA; CRYOPROTECTANT SYNTHESIS; FAT-BODY; PHOSPHORYLASE; METABOLISM; GLUCOSE; PIPIENS; KINASE; TISSUE;
Keywords:
RANA SYLVATICA; CRYOBIOLOGY; CRYOPRESERVATION; FREEZE TOLERANCE; LIVER METABOLISM; UREAGENESIS; GLYCOGEN PHOSPHORYLASE; PYRUVATE KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K.B. Storey e T.P. Mommsen, "EFFECTS OF TEMPERATURE AND FREEZING ON HEPATOCYTES ISOLATED FROM A FREEZE-TOLERANT FROG", The American journal of physiology, 266(5), 1994, pp. 180001477-180001482

Abstract

Metabolically active hepatocytes prepared from freeze-tolerant wood frogs, Rana sylvatica, were used to examine the direct effects of temperature and freezing on cryoprotectant synthesis and to assess the effectiveness of the natural cryoprotectant glucose in the freezing preservation of the isolated cells. Freshly isolated hepatocytes showed slowleakage of lactate dehydrogenase, readily synthesized urea, and oxidized a variety of C-14-labeled substrates. Effects of temperature on glucose production by isolated hepatocytes showed a normal Arrhenius relationship. However, compared with 0 degrees C control cells, either incubation at higher temperatures or freezing at -3 degrees C reduced the activity of glycogen phosphorylase alpha. These data suggest that the freezing-induced cryoprotectant production that occurs in vivo is not due to direct action of either low temperature or freezing on liver cell metabolism. The natural cryoprotectant glucose was also an excellent cryoprotectant of hepatocytes in vitro. In the absence of glucose,freezing caused a substantial leakage of lactate dehydrogenase from isolated hepatocytes, the rate of leakage increasing as freezing temperature decreased. Addition of 200-600 mM glucose to the incubation medium (similar to natural levels) fully protected cells against damage during freezing at -4 or -8 degrees C, normal freezing temperatures experienced by these frogs. Glucose also greatly improved freezing survival of isolated frog hepatocytes at ultralow temperatures (-80 or -196 degrees C).

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Documento generato il 02/12/20 alle ore 18:35:45