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Titolo:
TH1 AND TH2 CYTOKINE PRODUCTION BY PERIPHERAL-BLOOD MONONUCLEAR-CELLSFROM HIV-INFECTED PATIENTS
Autore:
BARCELLINI W; RIZZARDI GP; BORGHI MO; FAIN C; LAZZARIN A; MERONI PL;
Indirizzi:
IRCCS POLICLIN,PAD GRANELLI,IST MED INTERNA MALATTIE INFETT & IMMUNOPATOL,VIA F SFORZA 35 I-20122 MILAN ITALY UNIV MILAN,IRCCS,HOSP SAN RAFFAELE,INST INTERNAL MED INFECT DIS & IMMUNOPATHOL MILAN ITALY UNIV MILAN,IRCCS,HOSP SAN RAFFAELE,DEPT INFECT DIS MILAN ITALY
Titolo Testata:
AIDS
fascicolo: 6, volume: 8, anno: 1994,
pagine: 757 - 762
SICI:
0269-9370(1994)8:6<757:TATCPB>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN LYMPHOCYTES-B; DOWN-REGULATION; INTERLEUKIN-10; PATHOGENESIS; THYMOPENTIN; EXPRESSION; INVIVO; IL-10;
Keywords:
HIV INFECTION; TH1; TH2; INTERLEUKIN (IL)-2; INTERFERON-GAMMA; IL-4; IL-6; IL-10;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
W. Barcellini et al., "TH1 AND TH2 CYTOKINE PRODUCTION BY PERIPHERAL-BLOOD MONONUCLEAR-CELLSFROM HIV-INFECTED PATIENTS", AIDS, 8(6), 1994, pp. 757-762

Abstract

Objective: To study the TH1->TH2 cytokine switch, thought to occur during the progression of HIV infection. Design: We investigated interleukin (IL)-2, interferon (IFN)-gamma, IL-4, IL-6 and IL-10 production by phytohaemagglutinin (PHA)-stimulated and unstimulated peripheral blood mononuclear cell (PBMC) cultures from HIV-negative controls and HIV-positive subjects, stratified according to the Centers for Disease Control and Prevention (CDC) criteria. We correlated the above parameters with markers of disease progression. Methods: Cytokine production was measured in supernatants using enzyme-linked immunosorbent assay (ELISA) in 40 patients and 17 controls. To evaluate disease progression, we also determined CD4+ cell counts, PHA-induced proliferative response, p24 release and spontaneous immunoglobulin (Ig)G and IgM production. Results: In agreement with the TH1->TH2 switch hypothesis, we found that in the course of HIV disease mitogen-stimulated IL-2 production decreased, spontaneous and stimulated IL-6 production and spontaneous IL-10 secretion increased; IL-4 showed an increasing trend, although it was reduced in HIV-positive subjects. Finally, immunoglobulin production increased over time. In contrast, mitogen-stimulated IFN-gamma and IL-10 production did not change among the CDC categories, although theformer decreased and the latter increased in comparison with HIV-negative controls. Conclusions: Our data partially agree with the TH1->TH2switch hypothesis. Since IL-6 and IL-10 are produced by different cell types, whose proportions and functional features vary in the course of the disease, further experiments with purified lymphocyte subsets and monocytes are required. Nevertheless, as already suggested, we believe that a switch from a type 1 to a type 2 response occurs in HIV infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:30:52