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Titolo:
RAPID SEQUENCING OF OLIGONUCLEOTIDES BY HIGH-RESOLUTION MASS-SPECTROMETRY
Autore:
LITTLE DP; CHORUSH RA; SPEIR JP; SENKO MW; KELLEHER NL; MCLAFFERTY FW;
Indirizzi:
CORNELL UNIV,BAKER LAB,DEPT CHEM ITHACA NY 14853 CORNELL UNIV,BAKER LAB,DEPT CHEM ITHACA NY 14853
Titolo Testata:
Journal of the American Chemical Society
fascicolo: 11, volume: 116, anno: 1994,
pagine: 4893 - 4897
SICI:
0002-7863(1994)116:11<4893:RSOOBH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROSPRAY-IONIZATION; LARGE BIOMOLECULES; LARGE MOLECULES; OLIGODEOXYRIBONUCLEOTIDES; PROTEINS; DUPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
D.P. Little et al., "RAPID SEQUENCING OF OLIGONUCLEOTIDES BY HIGH-RESOLUTION MASS-SPECTROMETRY", Journal of the American Chemical Society, 116(11), 1994, pp. 4893-4897

Abstract

Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (less than or equal to 20 ppm) and resolving power (10(5)) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

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Documento generato il 04/07/20 alle ore 12:13:46